The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

Sep15  -  selenoprotein 15

Rattus norvegicus

Synonyms: 15 kDa selenoprotein
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of Sep15

  • Selenium deficiency is known to be associated with male infertility, and the selenoprotein PHGPx has been shown to increase in rat testis after puberty and to depend on gonadotropin stimulation in hypophysectomized rats [Roveri et al. (1992) J. Biol. Chem. 267, 6142 6146] [1].
  • These data may offer alternative explanations for the established toxicity of tellurium via disruption of selenoprotein function, particularly by the induction of intracellular oxidative stress by the inhibition of Se-dependent glutathione peroxidase [2].
  • These results suggest that ID-I is a selenoprotein and that Se deficiency can exacerbate the hypothyroidism observed in iodine deficiency [3].
  • We report the isolation and characterization of a new selenoprotein from a human lung adenocarcinoma cell line, NCI-H441 [4].
  • These results show that with the strategy utilized here, the capacity of selenoprotein synthesis in E. coli is more than sufficient for making possible the use of the bacteria for production of recombinant selenoproteins [5].

High impact information on Sep15


Chemical compound and disease context of Sep15


Biological context of Sep15

  • This report provides the first example of the ER-resident selenoprotein and suggests a possible role of the trace element selenium in the quality control of protein folding [10].
  • Pathogenesis of diquat-induced liver necrosis in selenium-deficient rats: assessment of the roles of lipid peroxidation and selenoprotein P [11].
  • Testosterone mediates expression of the selenoprotein PHGPx by induction of spermatogenesis and not by direct transcriptional gene activation [1].
  • During selenium deficiency there is a differential regulation of selenoprotein synthesis and gene expression; for example, there is a decrease in abundance of mRNA for cytosolic glutathione peroxidase (cGSH-Px) and a preservation of mRNA for phospholipid-hydroperoxide glutathione peroxidase (PHGSH-Px) [12].
  • The aim of the present work was to assess the role of cGSH-Px and PHGSH-Px 3' untranslated regions (UTRs) in the regulation of selenoprotein mRNA stability and translation by using H4-II-E-C3 cells transfected with different constructs containing a type I iodothyronine deiodinase-coding region linked to different selenoprotein mRNA 3' UTRs [12].

Anatomical context of Sep15


Associations of Sep15 with chemical compounds

  • Surprisingly, we found that GPX1 transcripts in Se deficiency are moderately abundant and similar in abundance to GAPDH and other selenoprotein mRNAs; Se supplementation increases GPX1 mRNA so that it is 30-fold higher than GAPDH mRNA [17].
  • The effect of dithiothreitol on secretion of selenoprotein P by H4IIE cells was examined [15].
  • These findings raise the possibility that selenoprotein P protects cell membranes against oxidant injury and that glutathione is involved in that protection [18].
  • In adult male rats, selenium deficiency results in a near complete loss in the selenoprotein 5'-deiodinase in the liver, resulting in decreased peripheral deiodination of thyroxine (T4) and increased serum T4 concentrations [19].
  • After the two columns were separated, selenoprotein P was eluted with heparin from heparin-Sepharose and albumin eluted from reactive blue with high salt [20].

Physical interactions of Sep15

  • SBP2 also interacts specifically with the minimally functional 3' UTR of another selenoprotein mRNA, deiodinase 1 [21].

Regulatory relationships of Sep15


Other interactions of Sep15


Analytical, diagnostic and therapeutic context of Sep15


  1. Testosterone mediates expression of the selenoprotein PHGPx by induction of spermatogenesis and not by direct transcriptional gene activation. Maiorino, M., Wissing, J.B., Brigelius-Flohé, R., Calabrese, F., Roveri, A., Steinert, P., Ursini, F., Flohé, L. FASEB J. (1998) [Pubmed]
  2. Binding of tellurium to hepatocellular selenoproteins during incubation with inorganic tellurite: consequences for the activity of selenium-dependent glutathione peroxidase. Garberg, P., Engman, L., Tolmachev, V., Lundqvist, H., Gerdes, R.G., Cotgreave, I.A. Int. J. Biochem. Cell Biol. (1999) [Pubmed]
  3. Inter-relationships between selenium and thyroid hormone metabolism in the rat and man. Beckett, G.J., Peterson, F.E., Choudhury, K., Rae, P.W., Nicol, F., Wu, P.S., Toft, A.D., Smith, A.F., Arthur, J.R. Journal of trace elements and electrolytes in health and disease. (1991) [Pubmed]
  4. A new selenoprotein from human lung adenocarcinoma cells: purification, properties, and thioredoxin reductase activity. Tamura, T., Stadtman, T.C. Proc. Natl. Acad. Sci. U.S.A. (1996) [Pubmed]
  5. High-level expression in Escherichia coli of selenocysteine-containing rat thioredoxin reductase utilizing gene fusions with engineered bacterial-type SECIS elements and co-expression with the selA, selB and selC genes. Arnér, E.S., Sarioglu, H., Lottspeich, F., Holmgren, A., Böck, A. J. Mol. Biol. (1999) [Pubmed]
  6. Recognition of UGA as a selenocysteine codon in type I deiodinase requires sequences in the 3' untranslated region. Berry, M.J., Banu, L., Chen, Y.Y., Mandel, S.J., Kieffer, J.D., Harney, J.W., Larsen, P.R. Nature (1991) [Pubmed]
  7. Dual function of the selenoprotein PHGPx during sperm maturation. Ursini, F., Heim, S., Kiess, M., Maiorino, M., Roveri, A., Wissing, J., Flohé, L. Science (1999) [Pubmed]
  8. Conserved nucleotide sequences in the open reading frame and 3' untranslated region of selenoprotein P mRNA. Hill, K.E., Lloyd, R.S., Burk, R.F. Proc. Natl. Acad. Sci. U.S.A. (1993) [Pubmed]
  9. Association of selenoprotein P with testosterone production in cultured Leydig cells. Nishimura, K., Matsumiya, K., Tsujimura, A., Koga, M., Kitamura, M., Okuyama, A. Arch. Androl. (2001) [Pubmed]
  10. Association between the 15-kDa selenoprotein and UDP-glucose:glycoprotein glucosyltransferase in the endoplasmic reticulum of mammalian cells. Korotkov, K.V., Kumaraswamy, E., Zhou, Y., Hatfield, D.L., Gladyshev, V.N. J. Biol. Chem. (2001) [Pubmed]
  11. Pathogenesis of diquat-induced liver necrosis in selenium-deficient rats: assessment of the roles of lipid peroxidation and selenoprotein P. Burk, R.F., Hill, K.E., Awad, J.A., Morrow, J.D., Kato, T., Cockell, K.A., Lyons, P.R. Hepatology (1995) [Pubmed]
  12. Role of the 3' untranslated region in the regulation of cytosolic glutathione peroxidase and phospholipid-hydroperoxide glutathione peroxidase gene expression by selenium supply. Bermano, G., Arthur, J.R., Hesketh, J.E. Biochem. J. (1996) [Pubmed]
  13. Nonsense-mediated decay of mRNA for the selenoprotein phospholipid hydroperoxide glutathione peroxidase is detectable in cultured cells but masked or inhibited in rat tissues. Sun, X., Li, X., Moriarty, P.M., Henics, T., LaDuca, J.P., Maquat, L.E. Mol. Biol. Cell (2001) [Pubmed]
  14. Selenoprotein mRNA is expressed in blood at levels comparable to major tissues in rats. Evenson, J.K., Wheeler, A.D., Blake, S.M., Sunde, R.A. J. Nutr. (2004) [Pubmed]
  15. Effect of selenium on selenoprotein P expression in cultured liver cells. Hill, K.E., Chittum, H.S., Lyons, P.R., Boeglin, M.E., Burk, R.F. Biochim. Biophys. Acta (1996) [Pubmed]
  16. Phospholipid hydroperoxide glutathione peroxidase of rat testis. Gonadotropin dependence and immunocytochemical identification. Roveri, A., Casasco, A., Maiorino, M., Dalan, P., Calligaro, A., Ursini, F. J. Biol. Chem. (1992) [Pubmed]
  17. Selenium regulation of transcript abundance and translational efficiency of glutathione peroxidase-1 and -4 in rat liver. Weiss Sachdev, S., Sunde, R.A. Biochem. J. (2001) [Pubmed]
  18. Liver and kidney necrosis in selenium-deficient rats depleted of glutathione. Burk, R.F., Hill, K.E., Awad, J.A., Morrow, J.D., Lyons, P.R. Lab. Invest. (1995) [Pubmed]
  19. Placental 5-deiodinase activity and fetal thyroid hormone economy are unaffected by selenium deficiency in the rat. Chanoine, J.P., Alex, S., Stone, S., Fang, S.L., Veronikis, I., Leonard, J.L., Braverman, L.E. Pediatr. Res. (1993) [Pubmed]
  20. Determination of the distribution of selenium between glutathione peroxidase, selenoprotein P, and albumin in plasma. Deagen, J.T., Butler, J.A., Zachara, B.A., Whanger, P.D. Anal. Biochem. (1993) [Pubmed]
  21. Purification, redox sensitivity, and RNA binding properties of SECIS-binding protein 2, a protein involved in selenoprotein biosynthesis. Copeland, P.R., Driscoll, D.M. J. Biol. Chem. (1999) [Pubmed]
  22. Dietary selenium intake controls rat plasma selenoprotein P concentration. Yang, J.G., Hill, K.E., Burk, R.F. J. Nutr. (1989) [Pubmed]
  23. Evidence that type III iodothyronine deiodinase in rat astrocyte is a selenoprotein. Ramauge, M., Pallud, S., Esfandiari, A., Gavaret, J., Lennon, A., Pierre, M., Courtin, F. Endocrinology (1996) [Pubmed]
  24. Dietary selenium requirements based on glutathione peroxidase-1 activity and mRNA levels and other Se-dependent parameters are not increased by pregnancy and lactation in rats. Sunde, R.A., Evenson, J.K., Thompson, K.M., Sachdev, S.W. J. Nutr. (2005) [Pubmed]
  25. Cloning and expression of a cDNA for a mammalian type III iodothyronine deiodinase. Croteau, W., Whittemore, S.L., Schneider, M.J., St Germain, D.L. J. Biol. Chem. (1995) [Pubmed]
  26. Purification and quantitation of a rat plasma selenoprotein distinct from glutathione peroxidase using monoclonal antibodies. Yang, J.G., Morrison-Plummer, J., Burk, R.F. J. Biol. Chem. (1987) [Pubmed]
  27. A protein binds the selenocysteine insertion element in the 3'-UTR of mammalian selenoprotein mRNAs. Hubert, N., Walczak, R., Carbon, P., Krol, A. Nucleic Acids Res. (1996) [Pubmed]
  28. Separation of selenium-containing proteins in human and mouse plasma using tandem high-performance liquid chromatography columns coupled with inductively coupled plasma-mass spectrometry. Koyama, H., Omura, K., Ejima, A., Kasanuma, Y., Watanabe, C., Satoh, H. Anal. Biochem. (1999) [Pubmed]
  29. Chemoprevention with triphenylselenonium chloride in selenium-deficient rats. Ip, C., Lisk, D.J., Ganther, H.E. Anticancer Res. (2000) [Pubmed]
  30. A comparison of the effects of dietary selenium on selenoprotein expression in rat brain and liver. Buckman, T.D., Sutphin, M.S., Eckhert, C.D. Biochim. Biophys. Acta (1993) [Pubmed]
WikiGenes - Universities