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Gene Review

Pik3cb  -  phosphatidylinositol-4,5-bisphosphate 3...

Rattus norvegicus

Synonyms: PI3-kinase subunit beta, PI3K-beta, PI3Kbeta, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta, Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform, ...
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Disease relevance of Pik3cb

  • A PI3K Pathway Mediates Hair Cell Survival and Opposes Gentamicin Toxicity in Neonatal Rat Organ of Corti [1].
  • Inhibition of hypoxia/reoxygenation-induced oxidative stress in HGF-stimulated antiapoptotic signaling: role of PI3-K and Akt kinase upon rac1 [2].
  • Co-infection of hepatocytes with recombinant adenoviruses to express dominant- negative forms of PI3 kinase (p110alpha/p110gamma) increased basal GSK3 activity, blocked the abilities of glucose and HGF treatments to inhibit GSK3 activity, and reduced basal c-Jun DNA binding [3].
  • Antisense-mediated suppression of the transmembrane protein-tyrosine phosphatase (PTPase) LAR has been shown previously to increase insulin-dependent phosphatidylinositol 3-kinase (PI 3-kinase) activation by greater than 300% in the rat hepatoma cell line McA-RH7777 [4].
  • Inhibition of the phosphatidyl inositol (PI) 3-kinase intermediate in IGF-I-mediated signal transduction confirmed that the PI 3-kinase pathway is required only at early stages for IGF-I-mediated hypertrophy and neonatal MyHC induction in these cells [5].

Psychiatry related information on Pik3cb

  • We conclude that the simultaneous inhibition of PI3K and PKC can induce GSK-3 overactivation, and further strengthen and prolong the Alzheimer-like tau hyperphosphorylation in N2a cells, suggesting the establishment of a cell model with early pathological events of Alzheimer's disease [6].

High impact information on Pik3cb

  • Here, we report that selection of the future axon among neurites of a cultured hippocampal neuron requires the activity of growth factor receptor tyrosine kinase, phosphatidylinositol 3-kinase (PI 3-kinase), as well as atypical protein kinase C (aPKC) [7].
  • Thus, neuronal polarity is likely to be controlled by the mPar3/mPar6/aPKC complex and the PI 3-kinase signaling pathway, both serving evolutionarily conserved roles in specifying cell polarity [7].
  • Role of substrates and products of PI 3-kinase in regulating activation of Rac-related guanosine triphosphatases by Vav [8].
  • Exocytosis of insulin promotes insulin gene transcription via the insulin receptor/PI-3 kinase/p70 s6 kinase and CaM kinase pathways [9].
  • The mutations S574A and S574E in Ca(v)beta(2a) prevented and mimicked, respectively, the effect of PI3K/Akt-PKB, indicating that phosphorylation of Ser574 on Ca(v)beta(2a) is necessary and sufficient to promote Ca(v) channel trafficking [10].

Chemical compound and disease context of Pik3cb

  • Dominant negative mutant of Akt/PKB or antioxidants, but not the dominant negative form of PI3-K, inhibited Ang II-induced protein synthesis and cell hypertrophy [11].
  • We demonstrated that both betaE2 and 17alpha-estradiol (alphaE2) significantly protected against H(2)O(2)-induced retinal neuron degeneration; however, progesterone had no effect. betaE2 transiently increased the phosphoinositide 3-kinase (PI3K) activity, when phosphoinositide 4,5-bisphosphate and [(32)gammaATP] were used as substrate [12].
  • However, both wortmannin, a PI3K inhibitor, and rapamycin, an inhibitor of p70(S6K) activity, inhibit 8,12-iso-iPF2alpha-III -induced myocyte hypertrophy, with IC50 values of 60 +/- 12 and 3 +/- 1.7 nM, respectively, whereas neither compound abrogates the PGF2alpha-mediated response [13].
  • To explore the significance of the PI3-kinase-Akt pathway, this study used PI3-kinase inhibitors (Wortmannin and LY294002) and recombinant adenoviruses encoding a dominant-active mutant of Akt (AxCAmyrAkt) and a dominant-negative mutant of Akt (AxCAAkt-AA) in cultured rat mesangial cells [14].
  • Although treatment with LY294002, a specific inhibitor of PI3 K, decreased the phosphorylation of Akt and increased Fkhr translocation to the nucleus, these events were not sufficient to induce FasL expression and apoptosis of C6 glioma cells [15].

Biological context of Pik3cb


Anatomical context of Pik3cb

  • Our results suggest that in rat adipocytes, sennidin A stimulates glucose incorporation in the phosphatidylinositol 3-kinase (PI3K)- and Akt-dependent, but in the IR/IRS1-independent manner [21].
  • The results indicate that PI3K promotes hair cell survival via its downstream targets, PKC and PKB/Akt [1].
  • In this study, we have investigated the in vivo mechanism of PI3K activation in the rodent retina and report the novel finding that light stimulates tyrosine phosphorylation of the beta-subunit of the insulin receptor (IRbeta) in ROS membranes, which leads to the association of PI3K enzyme activity with IRbeta [16].
  • Recently, we have shown that phosphoinositide 3-kinase (PI3K) in bovine rod outer segment (ROS) is activated in vitro by tyrosine phosphorylation of the C-terminal tail of the insulin receptor (Rajala, R. V. S., and Anderson, R. E. (2001) Invest. Ophthal. Vis. Sci. 42, 3110-3117) [16].
  • Wortmanin, which specifically inhibits PI 3-kinase, was found to abolish the hepatocyte DNA synthetic response due to stimulation with dHGF [22].

Associations of Pik3cb with chemical compounds

  • The resultant chimeric p65(Tpr-Met) protein is constitutively phosphorylated on tyrosine residues in vivo and associates with a number of SH2-containing signaling molecules including the p85 subunit of PI-3 kinase and the Grb2 adaptor protein, which couples receptor tyrosine kinases to the Ras signaling pathway [23].
  • The data presented in this report show that in renal cells there is a spatial separation of the inositol lipid signalling system between BLM and BBM, and that HGF causes activation of PLC and PI3K primarily in BLM, which leads to calpain-mediated activation of PI3K-C2 beta in BBM with a concomitant increase in PtdIns3P [24].
  • When exposed to gentamicin with the PI3K inhibitor LY294002 (10, 50 muM), the protein kinase C (PKC) inhibitor calphostin C (50, 100 nM) or the PKB/Akt inhibitor SH-6 (5, 10 muM), hair cell damage was significantly increased compared to gentamicin alone [1].
  • In pure preparations of cholangiocytes, we examined the effect of taurocholate (in the absence or presence of wortmannin or PI 3,4-bisphosphate the lipid product of PI3-K) on cholangiocyte proliferation and secretin-stimulated cyclic adenosine 3',5'-monophosphate (cAMP) levels [25].
  • CONCLUSIONS: Bile acid uptake by ABAT and the PI3-K pathway are important for bile acids to signal cholangiocyte proliferation [25].

Other interactions of Pik3cb

  • In this work, we investigated the role of 2 main signaling pathways, phosphoinositide 3-kinase (PI3K) and mitogen-activated protein kinase (MEK)/extracellular signal-regulated kinase (ERK), in these processes [26].
  • T3 treatment rapidly increased PI3K activity by 52 +/- 3% (p < 0.005), which resulted in increased phosphorylation of downstream kinases Akt and mammalian target of rapamycin (mTOR) [27].
  • Thus, rapid T3-mediated activation of PI3K by cytosolic TRalpha1 and subsequent activation of the Akt-mTOR-S6K signaling pathway may underlie one of the mechanisms by which thyroid hormone regulates physiological cardiac growth [27].
  • CONCLUSION: Induction of apoptosis during IR liver injury might be triggered by inactivation of the antiapoptotic PI3-K-PKB pathway and activation of the proapoptotic MAPKs [28].
  • PI3-K, PKB, BAD and MAPK activities were measured in ischaemic and non-ischaemic lobes at various times after reperfusion [28].

Analytical, diagnostic and therapeutic context of Pik3cb

  • Co-immunoprecipitation studies showed a direct interaction of cytosol-localized thyroid hormone receptor TRalpha1 and the p85alpha subunit of PI3K [27].
  • Rat neutrophils expressed both class IA PI3K subunits (p85, p110alpha, p110beta, and p110delta) and a class IB PI3K subunit (p110gamma) as assessed by a combination of Western blotting and reverse transcription-polymerase chain reaction (RT-PCR) approaches [29].
  • Here we investigated the role of the phosphatidylinositol 3 kinase (PI3K) signal transduction pathway in long-lasting behavioral sensitization to cocaine in rats, an animal model of the long-lasting functional changes induced by repeated drug use [30].
  • Using interference reflection microscopy, we found that activation of phosphoinositide 3-kinase (PI 3-kinase) by PDGF induces the dissipation of focal adhesions [31].
  • The present study is designed to test whether phosphatidylinositol 3-kinase (PI3-kinase) has a role in the signaling pathway in ischemic preconditioning (PC) and whether it is proximal or distal to protein kinase C (PKC) [32].


  1. A PI3K Pathway Mediates Hair Cell Survival and Opposes Gentamicin Toxicity in Neonatal Rat Organ of Corti. Chung, W.H., Pak, K., Lin, B., Webster, N., Ryan, A.F. J. Assoc. Res. Otolaryngol. (2006) [Pubmed]
  2. Inhibition of hypoxia/reoxygenation-induced oxidative stress in HGF-stimulated antiapoptotic signaling: role of PI3-K and Akt kinase upon rac1. Ozaki, M., Haga, S., Zhang, H.Q., Irani, K., Suzuki, S. Cell Death Differ. (2003) [Pubmed]
  3. The Ras/Rac1/Cdc42/SEK/JNK/c-Jun cascade is a key pathway by which agonists stimulate DNA synthesis in primary cultures of rat hepatocytes. Auer, K.L., Contessa, J., Brenz-Verca, S., Pirola, L., Rusconi, S., Cooper, G., Abo, A., Wymann, M.P., Davis, R.J., Birrer, M., Dent, P. Mol. Biol. Cell (1998) [Pubmed]
  4. The transmembrane protein-tyrosine phosphatase LAR modulates signaling by multiple receptor tyrosine kinases. Kulas, D.T., Goldstein, B.J., Mooney, R.A. J. Biol. Chem. (1996) [Pubmed]
  5. Maturation of the myogenic program is induced by postmitotic expression of insulin-like growth factor I. Musarò, A., Rosenthal, N. Mol. Cell. Biol. (1999) [Pubmed]
  6. Prolonged Alzheimer-like tau hyperphosphorylation induced by simultaneous inhibition of phosphoinositol-3 kinase and protein kinase C in N2a cells. Xu, G.G., Deng, Y.Q., Liu, S.J., Li, H.L., Wang, J.Z. Acta Biochim. Biophys. Sin. (Shanghai) (2005) [Pubmed]
  7. Hippocampal neuronal polarity specified by spatially localized mPar3/mPar6 and PI 3-kinase activity. Shi, S.H., Jan, L.Y., Jan, Y.N. Cell (2003) [Pubmed]
  8. Role of substrates and products of PI 3-kinase in regulating activation of Rac-related guanosine triphosphatases by Vav. Han, J., Luby-Phelps, K., Das, B., Shu, X., Xia, Y., Mosteller, R.D., Krishna, U.M., Falck, J.R., White, M.A., Broek, D. Science (1998) [Pubmed]
  9. Exocytosis of insulin promotes insulin gene transcription via the insulin receptor/PI-3 kinase/p70 s6 kinase and CaM kinase pathways. Leibiger, I.B., Leibiger, B., Moede, T., Berggren, P.O. Mol. Cell (1998) [Pubmed]
  10. PI3K promotes voltage-dependent calcium channel trafficking to the plasma membrane. Viard, P., Butcher, A.J., Halet, G., Davies, A., Nürnberg, B., Heblich, F., Dolphin, A.C. Nat. Neurosci. (2004) [Pubmed]
  11. Angiotensin II activates Akt/protein kinase B by an arachidonic acid/redox-dependent pathway and independent of phosphoinositide 3-kinase. Gorin, Y., Kim, N.H., Feliers, D., Bhandari, B., Choudhury, G.G., Abboud, H.E. FASEB J. (2001) [Pubmed]
  12. Involvement of insulin/phosphoinositide 3-kinase/Akt signal pathway in 17 beta-estradiol-mediated neuroprotection. Yu, X., Rajala, R.V., McGinnis, J.F., Li, F., Anderson, R.E., Yan, X., Li, S., Elias, R.V., Knapp, R.R., Zhou, X., Cao, W. J. Biol. Chem. (2004) [Pubmed]
  13. Prostaglandin F2alpha (PGF2alpha) and the isoprostane, 8, 12-iso-isoprostane F2alpha-III, induce cardiomyocyte hypertrophy. Differential activation of downstream signaling pathways. Kunapuli, P., Lawson, J.A., Rokach, J.A., Meinkoth, J.L., FitzGerald, G.A. J. Biol. Chem. (1998) [Pubmed]
  14. The PI3-kinase-Akt pathway promotes mesangial cell survival and inhibits apoptosis in vitro via NF-kappa B and Bad. Shimamura, H., Terada, Y., Okado, T., Tanaka, H., Inoshita, S., Sasaki, S. J. Am. Soc. Nephrol. (2003) [Pubmed]
  15. Inhibition of Akt kinase signalling and activation of Forkhead are indispensable for upregulation of FasL expression in apoptosis of glioma cells. Ciechomska, I., Pyrzynska, B., Kazmierczak, P., Kaminska, B. Oncogene (2003) [Pubmed]
  16. In vivo regulation of phosphoinositide 3-kinase in retina through light-induced tyrosine phosphorylation of the insulin receptor beta-subunit. Rajala, R.V., McClellan, M.E., Ash, J.D., Anderson, R.E. J. Biol. Chem. (2002) [Pubmed]
  17. Hepatocyte growth factor protects cultured rat cerebellar granule neurons from apoptosis via the phosphatidylinositol-3 kinase/Akt pathway. Zhang, L., Himi, T., Morita, I., Murota, S. J. Neurosci. Res. (2000) [Pubmed]
  18. Liver cell proliferation requires methionine adenosyltransferase 2A mRNA up-regulation. Pañeda, C., Gorospe, I., Herrera, B., Nakamura, T., Fabregat, I., Varela-Nieto, I. Hepatology (2002) [Pubmed]
  19. Epigallocathechin-3 gallate inhibits cardiac hypertrophy through blocking reactive oxidative species-dependent and -independent signal pathways. Li, H.L., Huang, Y., Zhang, C.N., Liu, G., Wei, Y.S., Wang, A.B., Liu, Y.Q., Hui, R.T., Wei, C., Williams, G.M., Liu, D.P., Liang, C.C. Free Radic. Biol. Med. (2006) [Pubmed]
  20. Hepatocyte growth factor induces retinal vascular permeability via MAP-kinase and PI-3 kinase without altering retinal hemodynamics. Clermont, A.C., Cahill, M., Salti, H., Rook, S.L., Rask-Madsen, C., Goddard, L., Wong, J.S., Bursell, D., Bursell, S.E., Aiello, L.P. Invest. Ophthalmol. Vis. Sci. (2006) [Pubmed]
  21. Sennidin stimulates glucose incorporation in rat adipocytes. Abe, D., Saito, T., Sekiya, K. Life Sci. (2006) [Pubmed]
  22. Hepatocyte growth factor-induced proliferation of primary hepatocytes is mediated by activation of phosphatidylinositol 3-kinase. Skouteris, G.G., Georgakopoulos, E. Biochem. Biophys. Res. Commun. (1996) [Pubmed]
  23. Activation of the JNK pathway is essential for transformation by the Met oncogene. Rodrigues, G.A., Park, M., Schlessinger, J. EMBO J. (1997) [Pubmed]
  24. Hepatocyte growth factor activates phosphoinositide 3-kinase C2 beta in renal brush-border plasma membranes. Crljen, V., Volinia, S., Banfic, H. Biochem. J. (2002) [Pubmed]
  25. Bile acid depletion and repletion regulate cholangiocyte growth and secretion by a phosphatidylinositol 3-kinase-dependent pathway in rats. Alpini, G., Glaser, S., Alvaro, D., Ueno, Y., Marzioni, M., Francis, H., Baiocchi, L., Stati, T., Barbaro, B., Phinizy, J.L., Mauldin, J., Lesage, G. Gastroenterology (2002) [Pubmed]
  26. PI3K-FRAP/mTOR pathway is critical for hepatocyte proliferation whereas MEK/ERK supports both proliferation and survival. Coutant, A., Rescan, C., Gilot, D., Loyer, P., Guguen-Guillouzo, C., Baffet, G. Hepatology (2002) [Pubmed]
  27. Thyroid hormone stimulates protein synthesis in the cardiomyocyte by activating the Akt-mTOR and p70S6K pathways. Kenessey, A., Ojamaa, K. J. Biol. Chem. (2006) [Pubmed]
  28. Involvement of protein kinase B and mitogen-activated protein kinases in experimental normothermic liver ischaemia-reperfusion injury. Cursio, R., Filippa, N., Miele, C., Van Obberghen, E., Gugenheim, J. The British journal of surgery. (2006) [Pubmed]
  29. Activation of phosphoinositide 3-kinase and Src family kinase is required for respiratory burst in rat neutrophils stimulated with artocarpol A. Kuan, Y.H., Lin, R.H., Lin, H.Y., Huang, L.J., Tsai, C.R., Tsao, L.T., Lin, C.N., Chang, L.C., Wang, J.P. Biochem. Pharmacol. (2006) [Pubmed]
  30. Neural plasticity and addiction: PI3-kinase and cocaine behavioral sensitization. Izzo, E., Martin-Fardon, R., Koob, G.F., Weiss, F., Sanna, P.P. Nat. Neurosci. (2002) [Pubmed]
  31. Restructuring of focal adhesion plaques by PI 3-kinase. Regulation by PtdIns (3,4,5)-p(3) binding to alpha-actinin. Greenwood, J.A., Theibert, A.B., Prestwich, G.D., Murphy-Ullrich, J.E. J. Cell Biol. (2000) [Pubmed]
  32. Ischemic preconditioning activates phosphatidylinositol-3-kinase upstream of protein kinase C. Tong, H., Chen, W., Steenbergen, C., Murphy, E. Circ. Res. (2000) [Pubmed]
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