Disease relevance of Nupr1

• Glucotoxicity in the INS-1 Rat Insulinoma Cell Line Is Mediated by the Orphan Nuclear Receptor Small Heterodimer Partner [1].
• METHODS: Nuclear cataracts were produced by culturing lenses from 4-week-old mice and rats in calcium ionophore A23187 or by injection of buthionine sulfoximine (BSO) into 7-day-old mice [2].
• Male Sprague-Dawley rats were kindled from the left amygdala until they exhibited Racine's class 5 generalized seizures [Racine (1972). EMBO J. 11, 3337-3346] Nuclear proteins were extracted from dorsal hippocampi obtained from 0 to 24 h after final kindling stimulation [3].
• 31P Nuclear magnetic resonance spectroscopy has been used to investigate the combined effect of 70 mM ethanol administration and hypoxia on the energetic metabolism and cytosolic pH of the perfused liver isolated from naive, fasted or chronically ethanol-treated rats [4].
• BACKGROUND: Nuclear localizing beta-galactosidase (nls beta-gal) is used as a marker for studying myoblast cell lineage and for evaluating myoblast survival after myoblast transfer, a procedure with potential use for gene complementation for muscular dystrophy [5].

Psychiatry related information on Nupr1

• The effect of exposure to the magnetic and radio-frequency fields associated with Nuclear Magnetic Resonance Imaging (MRI) on two standard animal behavior tests, was examined in rats [6].

High impact information on Nupr1

• (i) Nuclear matrix attachment elements in the rat OC gene promoter that bind nuclear matrix proteins with sequence specificity may impose structural constraints on promoter conformation [7].
• Ca2+- and Protein Kinase C-dependent Signaling Pathway for Nuclear Factor-{kappa}B Activation, Inducible Nitric-oxide Synthase Expression, and Tumor Necrosis Factor-{alpha} Production in Lipopolysaccharide-stimulated Rat Peritoneal Macrophages [8].
• Induction of Neutrophil Gelatinase-Associated Lipocalin in Vascular Injury via Activation of Nuclear Factor-{kappa}B [9].
• Free Fatty Acids Produce Insulin Resistance and Activate the Proinflammatory Nuclear Factor-{kappa}B Pathway in Rat Liver [10].
• Nuclear protein p8 is associated with glucose-induced pancreatic beta-cell growth [11].

Chemical compound and disease context of Nupr1

• The Expression of Nuclear Factor kappa B in Inflammation-Induced Rat Corneal Neovascularization [12].
• METHODS AND RESULTS: Nuclear staining with Hoechst 33258 or propidium iodine showed an increase in apoptotic cells with activation of caspase-3 in VSMCs infected with adenovirus expressing the dominant-negative form of CREB (AdCREBM1) [13].
• Reversal Effect of Thalidomide on Established Hepatic Cirrhosis in Rats via Inhibition of Nuclear Factor-kappaB/Inhibitor of Nuclear Factor-kappaB Pathway [14].

Biological context of Nupr1

• Exposure to serum-free medium (i) sensitized astrocytes to oxidative stress, (ii) reduced the expression of several genes involved in protection against oxidative stress, including heme oxygenase 1, and (iii) changed the expression of several genes involved in the control of cell survival, including the stress-regulated protein p8 [15].
• As a model, we used phosphodiester dumbbell oligonucleotides containing a binding site for the liver-enriched transcription factor HNF-1 (Hepatocyte Nuclear Factor 1) [16].
• BACKGROUND/AIMS: Nuclear translocation and DNA binding of NF-kappaB is essential, as interleukin-1beta (IL-1beta) stimulates the induction of inducible nitric oxide synthase (iNOS) gene expression in hepatocytes [17].
• METHODS: Nuclear magnetic resonance techniques were used in an isolated, perfused rat heart model of acute hibernation to determine the changes in metabolites and creatine kinase kinetics [18].
• Dissociation kinetics verified the necessity of an extended incubation (86 h) for maximum exchange at 4 degrees C. Nuclear androgen receptor concentration per mg DNA decreased between 15 and 25 days of age, from 375 to 146 fmol per mg DNA, then increased to 584 fmol per mg DNA at 90 days [19].

Anatomical context of Nupr1

• 2) Nuclear and cytoplasmic swelling were prominent responses in the caudoputamen and cerebral cortex at a time when neurons showed minimal alterations [20].
• Unoccupied 1,25-dihydroxyvitamin D3 receptors. Nuclear/cytosol ratio depends on ionic strength [21].
• Quantitative cytological analysis of functional changes in adrenomedullary chromaffin cells in normal, sham-operated, and pinealectomized rats in relation to time of day: III. Nuclear density [22].
• 1. Nuclear, nuclear-envelope and microsomal preparations were prepared from rat liver, and their purity and morphology monitored by electron microscopy [23].
• We measured transcription factor Nuclear Factor kappa B by electrophoretic mobility shift assay and Western analysis in young and aged rat brain tissues and report that basal levels of Nuclear Factor kappa B DNA-binding activity increase in the hippocampus and basal forebrain with age to significantly higher levels at 30 months of age [24].

Associations of Nupr1 with chemical compounds

• METHODS: Nuclear extracts were prepared from rat HSCs isolated and cultured from normal and carbon tetrachloride injured rat livers and from HSCs isolated from human liver [25].
• Effect of allylisopropylacetamide on Nuclear Ribonucleic Acid synthesis in rat liver [26].
• 3. Nuclear uracil-DNA glycosylase catalyzes the release of free uracil from both single-stranded and double-stranded DNA with the former being the preferred substrate [27].
• 3. Nuclear preparations resemble activated rather than native microsomal preparations in failing to transfer glucuronic acid from 4-nitrophenyl glucuronide to 2-aminophenol [23].
• We warn Nuclear Medicine scientists against the use of colloid uptake measurement as a marker of the bone macrophagic population, especially in situations associated with modified bone plasma flow [28].

Regulatory relationships of Nupr1

• Tumor protein p53-induced nuclear protein 1 (TP53INP1) in spontaneous chronic pancreatitis in the WBN/Kob rat: drug effects on its expression in the pancreas [29].
• TNF-alpha strongly activates the canonical Nuclear Factor Kappa-B (NF- kappaB) pathway [30].

Other interactions of Nupr1

• AIM: Nuclear factor kappa B (NF-kappaB) regulates a large number of genes involved in the inflammatory response to critical illnesses, but it is not known if and how NF-kappaB is activated and intercellular adhesion molecule-1 (ICAM-1) expressed in the gut following traumatic brain injury (TBI) [31].
• The level of Nuclear Factor (kappa) inhibitor (IkappaBalpha) in liver was decreased and DNA-binding activity of Nuclear Factor-kappaB (NF-kappaB) was increased after thermal injury. p38 MAP kinase was more significantly activated in liver harvested from burn rats than from shams [32].
• RESULTS: Nuclear extract isolated from obstructed bladders showed intense activator protein-1 binding activity 3, 6 and 24 hours after obstruction as well as increased nuclear factor-kappaB binding activity after 6 and 24 hours [33].
• Heat stressing stimulates nuclear protein kinase C raising diacylglycerol levels. Nuclear protein kinase C activation precedes Hsp70 mRNA expression [34].

Analytical, diagnostic and therapeutic context of Nupr1

• METHODS: Nuclear protein bound to the lactase gene cis element, CE-LPH1, was analyzed by electrophoretic mobility shift assays and supershifts with Cdx2 antibody [35].
• In vivo assessment of liver mass in the rat was achieved using several parameters obtained by scintigraphy, including one computed using a Nuclear Data 50:50 analysis system [36].
• RESULTS: Nuclear factor kappaB-DNA binding activity was significantly increased at the end of reperfusion in rat myocardium, and cytosolic IkappaB-alpha was decreased [37].
• The ultrastructure of the accessory sex organs of the male rat. XI. Nuclear alterations of prostatic epithelial cells induced by castration [38].
• We described the use of Nuclear microscopy (microbeam PIXE) for the quantitative micron scale analysis of platinum based chemotherapeutic agents in individual cell and tissue slices [39].

References