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Gene Review

SEC14L2  -  SEC14-like 2 (S. cerevisiae)

Homo sapiens

Synonyms: Alpha-tocopherol-associated protein, C22orf6, KIAA1186, KIAA1658, SEC14-like protein 2, ...
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Disease relevance of SEC14L2

  • Here, we describe the identification, the molecular cloning into Escherichia coli, and the in vitro expression of the human homologue of bovine TAP, hTAP [1].
  • 4. Paired SPF measurements on the primary and relapsed breast carcinomas of 14 patients showed that the SPF usually increased over time [2].
  • Low SPF's were associated with older age, minimal nuclear anaplasia, and estrogen receptor positivity, but SPF was the only variable that could be shown to have independent prognostic significance [2].
  • A significant correlation (r = -0.48) was found between the logarithm of the S-phase fraction of the primary tumour (SPF) and the logarithm of the doubling time of lung metastases (T2) [3].
  • METHODS AND MATERIAL: SPF of 171 primary squamous cell carcinomas of the head and neck and 183 nodal negative breast carcinomas was determined by one-dimensional flow cytometry [4].

Psychiatry related information on SEC14L2

  • In households reading the comic book (N-122), respondents reported the following changes as a direct result of readership--avoidance of sun exposure between 10 am and 2 pm (44.3%), use of sunscreens with SPF 8 and over (38.5%), skin self-examination (34.4%), and use of protective clothing (29.5%) [5].

High impact information on SEC14L2

  • Here we show that RF2 is in an open conformation when bound to the ribosome, allowing GGQ to reach the PTC while still allowing SPF-stop-codon interaction [6].
  • HBX lines as well as clonal cell lines of cells transfected with 246p53 (246 cell lines), cotransfected with HBx and 246p53 (246x lines) or transfected with control plasmids, were analyzed by flow cytometry to determine the fraction of cells in S phase (SPF) [7].
  • Class I release factors 1 and 2 (RF1 and RF2) terminate protein synthesis by recognizing stop codons on the mRNA via their conserved amino acid motifs (NIKS in eRF1 and SPF in RF2) and by the conserved tripeptide (GGQ) interactions with the ribosomal peptidyltransferase center [8].
  • Although supernatant protein factor activity was reduced by more than one-half, it could not be restored by the addition of ATP or protein kinase C delta with ATP, suggesting that dietary cholesterol reduced the expression of this protein [9].
  • The mechanism of stimulation is poorly understood, although supernatant protein factor belongs to a family of lipid-binding proteins that includes Sec14p and alpha-tocopherol transfer protein [9].

Chemical compound and disease context of SEC14L2


Biological context of SEC14L2


Anatomical context of SEC14L2

  • Labeled SPF preparations, containing 1 to 2 modified lysine residues/molecule of protein which retained full biological activity, were found to bind only weakly to microsomes under a variety of experimental conditions as determined by sucrose density gradient centrifugation [14].
  • Addition of ATP to rat liver cytosol increased supernatant protein factor activity by more than 2-fold and could be prevented by the addition of inhibitors of protein kinases A and C. Incubation of purified recombinant supernatant protein factor with ATP and protein kinases A or C delta similarly increased activity by more than 2-fold [9].
  • Splenic T cells from SPF NC/Nga mice had a level of CD40 ligand (CD40L) expression comparable to that of BALB/c mice [15].
  • There are strong correlations between DNI and SPF (P = 0.0001) and between flow cytometry parameters and clinical and histopathological factors such as axillary lymph node involvement, tumour size and histological grade [16].
  • To better understand the function of CTGF during wound healing, normal porcine fibroblasts were isolated from skin samples from SPF Yorkshire pigs [17].

Associations of SEC14L2 with chemical compounds


Other interactions of SEC14L2


Analytical, diagnostic and therapeutic context of SEC14L2

  • The establishment of germ-free, gnotobiotic, and SPF animal colonies is a major component of any effort to develop suitable animal models for aging research [22].
  • The results suggest that DNA ploidy has no independent prognostic value in transitional-cell bladder cancer, whereas proliferation indices (SPF, mitotic index) are important prognostic factors [23].
  • Five subjects who had received a mean total dose of 3.49 J/cm2 of UVB within a 4-week period had a mean SPF of 8.01 and 5 subjects who had received a mean total dose of 20 J/cm2 of UVA with PUVA therapy within 2 weeks had a mean SPF of 2 [24].
  • LiPA results were highly concordant with sequence analysis of the SPF amplimer, genotype-specific PCR, and sequence analysis of amplimers generated by MY 09/11 primers [25].
  • There was no consistent pattern of change in SPF values during the first cycle of chemotherapy, either for patients who responded to treatment or for those whose tumours did not regress [26].


  1. A novel human tocopherol-associated protein: cloning, in vitro expression, and characterization. Zimmer, S., Stocker, A., Sarbolouki, M.N., Spycher, S.E., Sassoon, J., Azzi, A. J. Biol. Chem. (2000) [Pubmed]
  2. Relationships of S-phase fraction of breast carcinoma in relapse to duration of remission, estrogen receptor content, therapeutic responsiveness, and duration of survival. Meyer, J.S., Lee, J.Y. Cancer Res. (1980) [Pubmed]
  3. Growth rate of lung metastases and S-phase fraction as determined by flow cytometry from the primary tumour in 25 patients with bone or soft-tissue sarcomas. Blomqvist, C., Huuhtanen, R., Pan, Y., Wiklund, T., Tarkkanen, M., Virolainen, M., Tribukait, B. Br. J. Cancer (1996) [Pubmed]
  4. Prognostic value of S-phase fraction in head and neck squamous cell carcinomas and nodal negative breast carcinomas. Fietkau, R., Iro, H., Tulusan, A.H., Dressel, V., Altendorf-Hofmann, A., Sauer, R. Strahlentherapie und Onkologie : Organ der Deutschen Röntgengesellschaft ... [et al]. (1994) [Pubmed]
  5. Skin cancer comic book: evaluation of a public educational vehicle. Putnam, G.L., Yanagisako, K.L. Cancer Detect. Prev. (1982) [Pubmed]
  6. A cryo-electron microscopic study of ribosome-bound termination factor RF2. Rawat, U.B., Zavialov, A.V., Sengupta, J., Valle, M., Grassucci, R.A., Linde, J., Vestergaard, B., Ehrenberg, M., Frank, J. Nature (2003) [Pubmed]
  7. Analysis of the tumorigenicity of the X gene of hepatitis B virus in a nontransformed hepatocyte cell line and the effects of cotransfection with a murine p53 mutant equivalent to human codon 249. Oguey, D., Dumenco, L.L., Pierce, R.H., Fausto, N. Hepatology (1996) [Pubmed]
  8. Release factors eRF1 and RF2: a universal mechanism controls the large conformational changes. Ma, B., Nussinov, R. J. Biol. Chem. (2004) [Pubmed]
  9. Phosphorylation of supernatant protein factor enhances its ability to stimulate microsomal squalene monooxygenase. Singh, D.K., Mokashi, V., Elmore, C.L., Porter, T.D. J. Biol. Chem. (2003) [Pubmed]
  10. Supernatant protein factor and tocopherol-associated protein: an unexpected link between cholesterol synthesis and vitamin E (review). Porter, T.D. J. Nutr. Biochem. (2003) [Pubmed]
  11. Randomized placebo-controlled trial of metronidazole 1% cream with sunscreen SPF 15 in treatment of rosacea. Tan, J.K., Girard, C., Krol, A., Murray, H.E., Papp, K.A., Poulin, Y., Chin, D.A., Jeandupeux, D. Journal of cutaneous medicine and surgery. (2002) [Pubmed]
  12. Topical isotretinoin for melasma in Thai patients: a vehicle-controlled clinical trial. Leenutaphong, V., Nettakul, A., Rattanasuwon, P. Journal of the Medical Association of Thailand = Chotmaihet thangphaet. (1999) [Pubmed]
  13. Zidovudine toxicity to cats infected with feline leukemia virus. Haschek, W.M., Weigel, R.M., Scherba, G., DeVera, M.C., Feinmehl, R., Solter, P., Tompkins, M.B., Tompkins, W.A. Fundamental and applied toxicology : official journal of the Society of Toxicology. (1990) [Pubmed]
  14. Interactions of supernatant protein factor with components of the microsomal squalene epoxidase system. Binding of supernatant protein factor to anionic phospholipids. Caras, I.W., Friedlander, E.J., Bloch, K. J. Biol. Chem. (1980) [Pubmed]
  15. IgE hyperproduction through enhanced tyrosine phosphorylation of Janus kinase 3 in NC/Nga mice, a model for human atopic dermatitis. Matsumoto, M., Ra, C., Kawamoto, K., Sato, H., Itakura, A., Sawada, J., Ushio, H., Suto, H., Mitsuishi, K., Hikasa, Y., Matsuda, H. J. Immunol. (1999) [Pubmed]
  16. DNA ploidy and other results of DNA flow cytometry as prognostic factors in operable breast cancer: 10 year results of a randomised study. Gnant, M.F., Blijham, G., Reiner, A., Reiner, G., Reynders, M., Schutte, B., van Asche, C., Steger, G., Jakesz, R. Eur. J. Cancer (1992) [Pubmed]
  17. Recombinant connective tissue growth factor modulates porcine skin fibroblast gene expression. Wang, J.F., Olson, M.E., Ball, D.K., Brigstock, D.R., Hart, D.A. Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society. (2003) [Pubmed]
  18. Supernatant protein factor in complex with RRR-alpha-tocopherylquinone: a link between oxidized Vitamin E and cholesterol biosynthesis. Stocker, A., Baumann, U. J. Mol. Biol. (2003) [Pubmed]
  19. Ligand specificity in the CRAL-TRIO protein family. Panagabko, C., Morley, S., Hernandez, M., Cassolato, P., Gordon, H., Parsons, R., Manor, D., Atkinson, J. Biochemistry (2003) [Pubmed]
  20. Characterization of a human Sec14-like protein cDNA SEC14L3 highly homologous to human SPF/TAP. Ye, X., Ji, C., Yin, G., Tang, R., Zeng, L., Gu, S., Ying, K., Xie, Y., Zhao, R.C., Mao, Y. Mol. Biol. Rep. (2004) [Pubmed]
  21. Tocopherol-associated protein is a ligand-dependent transcriptional activator. Yamauchi, J., Iwamoto, T., Kida, S., Masushige, S., Yamada, K., Esashi, T. Biochem. Biophys. Res. Commun. (2001) [Pubmed]
  22. Necessity of a more standardized microbiological characterization of rodents for aging studies. Sebesteny, A. Neurobiol. Aging (1991) [Pubmed]
  23. Flow cytometry in comparison with mitotic index in predicting disease outcome in transitional-cell bladder cancer. Lipponen, P.K., Nordling, S., Eskelinen, M.J., Jauhiainen, K., Terho, R., Harju, E. Int. J. Cancer (1993) [Pubmed]
  24. Natural and artificial photoprotection. Cripps, D.J. J. Invest. Dermatol. (1981) [Pubmed]
  25. Development and clinical evaluation of a highly sensitive PCR-reverse hybridization line probe assay for detection and identification of anogenital human papillomavirus. Kleter, B., van Doorn, L.J., Schrauwen, L., Molijn, A., Sastrowijoto, S., ter Schegget, J., Lindeman, J., ter Harmsel, B., Burger, M., Quint, W. J. Clin. Microbiol. (1999) [Pubmed]
  26. DNA flow cytometry and response to preoperative chemotherapy for primary breast cancer. O'Reilly, S.M., Camplejohn, R.S., Rubens, R.D., Richards, M.A. Eur. J. Cancer (1992) [Pubmed]
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