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Gene Review

FN1  -  fibronectin 1

Macaca mulatta

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Disease relevance of FN1


High impact information on FN1

  • Recent studies have reported improved efficiency by including stromal cells (STR), or the fibronectin fragment CH-296 (FN), and various cytokines such as flt3 ligand (FLT) during ex vivo culture and transduction in nonhuman primates [5].
  • Antibodies to vascular basement membrane and matrix markers laminin, fibronectin, and collagen types I and VIII, and antisera recognizing cell cycle-specific nuclear proteins (cyclin, Ki-67, Mib-1) also were used [6].
  • METHODS: The impact of culture and transduction on rhesus macaque CD34+ peripheral blood stem cells (PBSCs) was assessed in the presence of FN and stimulatory cytokines [7].
  • In the current study, we investigated whether prolonged culture and transduction in the presence of the carboxy-terminal portion of fibronectin (FN) could maintain or expand retrovirally transduced repopulating hematopoietic stem cells (HSCs) [7].
  • Levels of FFN in amnion increase with the onset of labor in rhesus monkeys [8].

Biological context of FN1


Anatomical context of FN1

  • We conclude that GC differentially regulates FFN expression in epithelial and mesenchymal cells from amnion and placenta [8].
  • CONCLUSIONS: These results suggest that culture on FN support allows prolonged ex vivo maintenance and even expansion of transduced repopulating stem cells [7].
  • Cultured simian astrocytes expressed vimentin, laminin, and fibronectin [13].
  • As macrophages, they expressed in vitro CD68, CD64, CD14, CD11b, MHC class II, and fibronectin [13].
  • During this period the trophoblast cells in the proximal shell reorganized, forming strands of cells that were separated by bands of matrix immunoreactive for type IV collagen, laminin, and fibronectin [14].

Associations of FN1 with chemical compounds

  • Cells were maintained with and without dexamethasone (DEX), and levels of FFN in the conditioned media were determined by ELISA [8].
  • Aqueous humor fibronectin levels were determined in four monkeys after approximately 9.5 weeks of timolol + dorzolamide treatment [15].
  • Our findings indicate that timolol affects neither ciliary epithelial transport of ascorbate nor aqueous fibronectin levels [15].
  • Surfaces on the experimental sides were decalcified with citric acid, and after thorough rinsing, the inner aspect of the flaps and the roots were bathed with 1 ml of autologous plasma fibronectin in normal saline (400 micrograms m/ml) and the flaps sutured [10].
  • Immunoperoxidase staining showed that these structures contained major basement membrane components including laminin, heparan sulfate proteoglycan, type IV collagen, as well as fibronectin [16].

Regulatory relationships of FN1

  • In the absence of serum, VEGF stimulated proliferation of UEA-1 (+) cells plated on fibronectin but not collagen I, whereas in the presence of 10% fetal calf serum, both matrices supported VEGF-induced mitogenesis [17].

Other interactions of FN1


Analytical, diagnostic and therapeutic context of FN1


  1. Interaction of Borrelia burgdorferi with peripheral blood fibrocytes, antigen-presenting cells with the potential for connective tissue targeting. Grab, D.J., Lanners, H., Martin, L.N., Chesney, J., Cai, C., Adkisson, H.D., Bucala, R. Mol. Med. (1999) [Pubmed]
  2. Conjunctival transplantation. Autologous and homologous grafts. Weise, R.A., Mannis, M.J., Vastine, D.W., Fujikawa, L.S., Roth, A.M. Arch. Ophthalmol. (1985) [Pubmed]
  3. Histologic, morphometric, and biochemical evolution of vein bypass grafts in a nonhuman primate model. II. Modification of early changes by platelet inhibition with aspirin and dipyridamole. Boerboom, L.E., Olinger, G.N., Liu, T.Z., Rodriguez, E.R., Ferrans, V.J., Kissebah, A.H. J. Thorac. Cardiovasc. Surg. (1990) [Pubmed]
  4. Effect of fibronectin on healing of replanted teeth in monkeys: a histologic and autoradiographic study. Nasjleti, C.E., Caffesse, R.G., Castelli, W.A., Lopatin, D.E., Kowalski, C.J. Oral Surg. Oral Med. Oral Pathol. (1987) [Pubmed]
  5. Prolonged high-level detection of retrovirally marked hematopoietic cells in nonhuman primates after transduction of CD34+ progenitors using clinically feasible methods. Wu, T., Kim, H.J., Sellers, S.E., Meade, K.E., Agricola, B.A., Metzger, M.E., Kato, I., Donahue, R.E., Dunbar, C.E., Tisdale, J.F. Mol. Ther. (2000) [Pubmed]
  6. Immunohistochemical characterization of developing and mature primate retinal blood vessels. Gariano, R.F., Iruela-Arispe, M.L., Sage, E.H., Hendrickson, A.E. Invest. Ophthalmol. Vis. Sci. (1996) [Pubmed]
  7. The presence of the carboxy-terminal fragment of fibronectin allows maintenance of non-human primate long-term hematopoietic repopulating cells during extended ex vivo culture and transduction. Sellers, S.E., Tisdale, J.F., Agricola, B.A., Donahue, R.E., Dunbar, C.E. Exp. Hematol. (2004) [Pubmed]
  8. Cell type-specific regulation of fetal fibronectin expression in amnion: conservation of glucocorticoid responsiveness in human and nonhuman primates. Ma, Y., Lockwood, C.J., Bunim, A.L., Giussani, D.A., Nathanielsz, P.W., Guller, S. Biol. Reprod. (2000) [Pubmed]
  9. Distribution of laminin, type IV collagen, and fibronectin in the cell columns and trophoblastic shell of early macaque placentas. Blankenship, T.N., Enders, A.C., King, B.F. Cell Tissue Res. (1992) [Pubmed]
  10. Cell proliferation after flap surgery, root conditioning and fibronectin application. Caffesse, R.G., Smith, B.A., Nasjleti, C.E., Lopatin, D.E. J. Periodontol. (1987) [Pubmed]
  11. Nerve growth factor enhances peripheral nerve regeneration in non-human primates. Ahmed, Z., Brown, R.A., Ljungberg, C., Wiberg, M., Terenghi, G. Scandinavian journal of plastic and reconstructive surgery and hand surgery / Nordisk plastikkirurgisk forening [and] Nordisk klubb for handkirurgi. (1999) [Pubmed]
  12. Ultrastructural verification of anchoring role of lamina fibroreticularis of dental basement membrane in odontogenesis. Sawada, T., Inoue, S. Journal of electron microscopy. (1999) [Pubmed]
  13. Obtention and characterization of primary astrocyte and microglial cultures from adult monkey brains. Guillemin, G., Boussin, F.D., Croitoru, J., Franck-Duchenne, M., Le Grand, R., Lazarini, F., Dormont, D. J. Neurosci. Res. (1997) [Pubmed]
  14. Developmental changes in the cell columns and trophoblastic shell of the macaque placenta: an immunohistochemical study localizing type IV collagen, laminin, fibronectin and cytokeratins. Blankenship, T.N., King, B.F. Cell Tissue Res. (1993) [Pubmed]
  15. Studies on the mechanism of action of timolol and on the effects of suppression and redirection of aqueous flow on outflow facility. Kiland, J.A., Gabelt, B.T., Kaufman, P.L. Exp. Eye Res. (2004) [Pubmed]
  16. Basement membrane-like structures occurring on the surface of dental papilla mesenchymal cells during odontogenesis in the monkey Macaca fuscata. Sawada, T., Inoue, S. Eur. J. Oral Sci. (1998) [Pubmed]
  17. Isolation and culture of microvascular endothelial cells from the primate corpus luteum. Christenson, L.K., Stouffer, R.L. Biol. Reprod. (1996) [Pubmed]
  18. Restricted distribution of integrin beta 6 mRNA in primate epithelial tissues. Breuss, J.M., Gillett, N., Lu, L., Sheppard, D., Pytela, R. J. Histochem. Cytochem. (1993) [Pubmed]
  19. Co-distribution of von Willebrand factor and fibronectin in cultured rhesus endothelial cells. Lou, D.A., Hu, F.N. Histochem. J. (1987) [Pubmed]
  20. Distribution of extracellular matrix components during early embryonic development in the macaque. Peterson, P.E., Pow, C.S., Wilson, D.B., Hendrickx, A.G. Acta anatomica. (1993) [Pubmed]
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