Disease relevance of ND-8792

• Firm induration and erythema are observed within 18 to 24 h, and visualization of the reaction may be enhanced by intravenous injection of Pontamine sky blue dye [1].
• The ischaemic area was determined after an intravenous injection of pontamine sky blue dye 1 h before induction of cardiac arrest with potassium chloride [2].
• Vascular permeability on the back skin was measured by the local accumulation of Pontamine sky blue (PSB) after subcutaneous injection of LPS (400 microg site-1) from Salmonella typhimurium [3].
• The edema lesions were stained blue by the intravenous injection of Pontamine Sky Blue 6B dye, suggesting that PT caused increased vascular permeability, similarly to the permeability factor (PF) of cholera toxin [4].
• Synthesis of CSB was undertaken to produce a product greater than 98% pure and this compound was used to elucidate the possible mechanisms by which this class of structurally related compounds inhibits HIV-1 [5].

High impact information on ND-8792

• In contrast paramethasone was equally effective when given either 24 hr or 3 hr before harvest in suppressing the appearance of intravenously injected pontamine sky blue in the exudate [6].
• In temporal studies, the kinetics of addition of T30177 to infected cell cultures indicated that, like the known viral adsorption blocking agents dextran sulfate and Chicago sky blue, T30177 needed to be added to cells during or very soon after viral infection [7].
• The same three dyes were also substrates for rat liver microsomal azoreductase enzymes whereas Congo red, trypan blue and Chicago sky blue were shown to be inactive in a previous publication [8].
• The ability of rat liver microsomes from phenobarbitone pretreated animals to reduce the azo groups of amaranth, sunset yellow, congo red, trypan blue, chloramine sky blue FF and direct black 38 was measured spectrophotometrically in vitro [9].
• 2. Plasma leakage in mouse skin was measured by the local accumulation of Pontamine sky blue at the site of subcutaneous injection of LPS (Sal. typhimurium) [10].

Biological context of ND-8792

• Vascular permeability was assessed by extravasation of Pontamine sky blue [11].
• This experiment with 45CaCl2 produced discrete areas of decidualization, as shown by the pontamine sky blue reaction on day 5 of pseudopregnancy [12].
• Inhalation of X/XOD produced a marked Pontamine sky blue exudation in the trachea, main bronchus and lungs [13].
• In each experiment two to five active sites were marked with pontamine sky blue, deposited from the recording electrode ionophoretically and located histologically [14].
• Implantation sites were stained by injections of pontamine sky blue dye on day 8 of gestation, fifteen minutes before sacrifice by cervical dislocation [15].

Anatomical context of ND-8792

• Blood plasma permeation in this model was examined by means of a blue dye (pontamine sky blue), and histological observation was performed by light and transmission electron microscopy [16].
• Interneuron responses were most commonly recorded in the first 200 micrometer of the descending tract, and this position was confirmed by the injection of pontamine sky blue and the examination of frozen sections [17].
• Areas of uterus responding to the oil (as indicated by the Pontamine Sky Blue reaction) showed an influx of leucocytes into the endometrial stroma between 16 and 36 h after the application of the decidual stimulus [18].
• Vascular perfusion with a fluorescent dye, Pontamine sky blue, was combined with glyoxylic acid-induced catecholamine fluorescence to produce simultaneous staining of intracerebral blood vessels and catecholamine-containing cells and fibers [19].
• We were also able to elicit this activity in a whole organ (from lip to stomach, midline intact) preparation, whose oral cavity was filled with dye (pontamine sky-blue 3 mM, 50 microl), using each of the three types of stimulation [20].

Associations of ND-8792 with other chemical compounds

• The position of recorded neurons was marked by Pontamine sky blue ejected from the glass pipette microelectrode, and was identified on sections processed for NADPH diaphorase histochemistry which specifically stained cholinergic neurons [21].
• Rank order of potency was maintained relative to [(125)I]-ligand filtration assays, and the effects of the highly colored compounds, tartrazine and Chicago Sky Blue, were insignificant on the polarization signal up to a concentration of 1 microM [22].
• Triple-barreled glass microelectrodes were used, each barrel of which was filled with solution containing one of three different dyes (Fast Green FCF, Pontamine Sky Blue and Nigrosine) for differentially labeling the sites exhibiting different stimulus effects [23].
• The blockade was achieved by microinjections of 2% xylocaine, laced with Pontamine Sky Blue to identify sites of injections, from the tip of a penetrating microelectrode [24].
• AGEPC-induced vascular permeability increase in the skins of rats, guinea pigs and rabbits was assessed by the leaked dye method, in which the dye was extracted from the skin sites injected with AGEPC after intravenous injection of pontamine sky blue [25].

Gene context of ND-8792

• In pregnant mice, the appearance of Pontamine Sky Blue spots provided an earlier indication of implantation than did determination of total uterine extravascular 125I-labelled albumin accumulation [26].
• The sites of 22 respiratory neurones were marked in 4-day-old animals by injecting Pontamine Sky Blue. These neurones were distributed from 175 microns anterior to 525 microns posterior to the obex, from 225 to 450 microns lateral to the midline and from 175 to 425 microns deep to the ventral surface of the brain stem [27].
• The hydrogen peroxide-induced PSB exudation effect was attenuated was attenuated by pretreatment with catalase, although heat-inactivated catalase had no inhibitory effect [28].
• The X/XOD-induced increase in Pontamine sky-blue exudation was attenuated by pretreatment with inhaled catalase, but not by superoxide dismutase [13].
• Lysozyme injected i.p. into mice inhibits acetic acid (i.p.) induced leakage of Pontamine Sky Blue into the abdominal cavity [29].

Analytical, diagnostic and therapeutic context of ND-8792

• In vivo, a short segment of the border between the forepaw-lower jaw representations in rat S1 was mapped using standard electrophysiological methods and was visibly marked using iontophoresis of pontamine sky blue dye [30].
• The recording sites of nociceptive units were marked by the electrophoretic deposition of pontamine sky blue from the recording microelectrode, and subsequently identified histologically in cresyl-violet stained sections [31].
• Pontamine sky blue: a counterstain for background autofluorescence in fluorescence and immunofluorescence histochemistry [32].
• The successful implantation was checked by injection of Chicago Sky Blue 6B on the second day after embryo transfer [33].

References