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Gene Review

Neu1  -  neuraminidase 1

Rattus norvegicus

Synonyms: Lysosomal sialidase, N-acetyl-alpha-neuraminidase 1, Sialidase-1
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Disease relevance of Neu1


High impact information on Neu1


Chemical compound and disease context of Neu1


Biological context of Neu1

  • Age-specific changes in glycosylation of rat intestinal lactase-phlorizin hydrolase were analyzed using enzyme immunoprecipitated from microvillus membranes of suckling, weaning, and adult rats, and carbohydrate moieties were examined by lectin affinity binding, metabolic labeling, and neuraminidase treatment [13].
  • This proposed mechanism is indistinguishable from the autoinhibition phenomenon described for another galactophilic binding protein, the hepatic binding protein (HBP), which binds its own terminal galactosyl residues following neuraminidase treatment [4].
  • A distinct population of neuraminidase-resistant WGA binding sites was also found in axons directly proximal to the transport block [14].
  • To examine whether hepatic plasma membrane receptors for asialoglycoproteins are replaced or reutilized during endocytosis, receptors on the surface of isolated hepatocytes were uniquely labeled by neuraminidase treatment [15].
  • Neuraminidase treatment sufficient to cause greater than 90% loss of insulin stimulatable lipogenesis had no effect on 125I-insulin binding, tyrosine kinase activity of partially purified insulin receptors, insulin receptor phosphorylation in intact cells, or insulin-induced receptor internalization [16].

Anatomical context of Neu1

  • It is concluded that cerebellar granule cells differentiated in vitro possess one lysosomal sialidase and two plasma membrane sialidases, all of them active on ganglioside [17].
  • In contrast, brain lysosomal sialidase was not significantly altered by ethanol treatment, even though the major proportion of the brain sialidase activity was localized in the lysosomes [18].
  • However, specific bile acid binding is markedly decreased by low concentrations of proteolytic enzymes and is also decreased by the action of neuraminidase and phospholipases A and C. These results are consistent with the existence of a homogeneous bile acid receptor protein in liver surface membranes [19].
  • Human breast sections, like those of the rat, showed fluorescent labeling at the apical region of the epithelial cells; this labeling increased if the tissue had prior treatment with neuraminidase [20].
  • T. cruzi neuraminidase also removed sialic acid from adult human saphenous vein endothelial cells, as determined by both histochemical and metabolic labeling studies [7].

Associations of Neu1 with chemical compounds

  • When isoelectric focusing electrophoresis was carried out over a narrow range of pH (5-7), each of the apolipoprotein E isoforms of affected members was observed as a doublet, even after reduction of dimers of the protein with 2-mercaptoethanol and treatment with neuraminidase to minimize the content of sialylated forms of the protein [21].
  • The glycoprotein nature of these species was investigated using endoglycosidase F (endo F) and neuraminidase treatment and wheat germ agglutinin-agarose chromatography [22].
  • Experiments to block adhesion by pretreatment of cells with either neuraminidase or mucin show that the sialic acids-rich moiety is on the nerve cells, while its receptor is on the muscle fibers [23].
  • PCF binding at pH 1.8 at the nonmodified luminal cell surface is significantly diminished by neuraminidase treatment which, however, does not perceptibly reduce PCF binding at the higher pH levels [24].
  • WGA binding is not removed by prior digestion with neuraminidase and succinyl-WGA also binds the proximal cilium, suggesting a predominance of N-acetylglucosamine containing glycoconjugates [25].

Physical interactions of Neu1

  • Both the mature 250-kDa receptor and the neuraminidase-digested 245-kDa form specifically bound 125-I-IGF-II [26].
  • Digestion of covalently linked [125I]human (h) GH-receptor complexes with neuraminidase or endoglycosidase F reduced the mass of the principal hormone receptor complex from about 130 kilodaltons (kDa) to 120 and 110 kDa, respectively, suggesting that about 20% of the mass of the GH receptor of rat adipocytes consists of N-linked sialocarbohydrates [27].
  • Treatment of both enzymes with neuraminidase enhanced the binding of aminopeptidase to RCAI-Sepharose by 4-fold but did not alter the binding patterns of dipeptidyl peptidase IV [28].

Other interactions of Neu1

  • Desialation of RBL cell surface glycoproteins by neuraminidase treatment eliminated IL-4 release [29].
  • Treatment of AtT-20 cell membranes with neuraminidase from V. cholera was also able to greatly reduce the affinity of SRIF receptors for [125I]MK 678.(ABSTRACT TRUNCATED AT 400 WORDS)[30]
  • Unlike NGF, the neuraminidase does not react with the apoptosis-causing pan-neurotrophin receptor p75NTR [31].
  • As muscles develop, the extent of sialylation of muscle N-CAM decreases, and a 140-kD desialo form of N-CAM (generated by neuraminidase treatment) is replaced by a 125-kD form [32].
  • Chemical analysis of such nuclei from neuraminidase-treated cells confirmed significant elevation of GM1 above control levels, along with virtual absence of markers for plasma membrane and Golgi apparatus [33].

Analytical, diagnostic and therapeutic context of Neu1

  • 125I-AII binding to GBM was increased after treatment of these membranes with collagenase, slightly diminished with neuraminidase, and almost completely abolished with trypsin suggesting the proteic nature of the receptor [34].
  • Such antibodies to tachyzoite proteins of < or = 14, 22, 26-28, 30, 46, 60, 70-80, and > 100 kD (eliminated by protease but not periodate or neuraminidase treatment) were demonstrated in whey from acutely infected subjects when Western blots were probed with their whey and antibodies to human secretory IgA or IgA or secretory piece [35].
  • Peanut agglutinin, purified by affinity chromatography, agglutinates lymphocytes from mouse, rat, guinea pig, and man only after their treatment with neuraminidase [36].
  • A 150-kDa C3b-binding protein was isolated from rat platelets, which had immunochemical and biochemical identity to plasma factor H. Immunofluorescence microscopy and flow cytometry demonstrated that factor H was present on the surface of rat and mouse platelets, which could be removed by treatment with neuraminidase [37].
  • However, two-dimensional gel electrophoresis in combination with neuraminidase digestion demonstrated that sialylation was markedly inhibited by Tris [38].


  1. Distinguishing mammalian sialidases by inhibition kinetics with novel derivatives of 5-acetamido-2,6-anhydro-3,5-dideoxy-D-glycero-D-galacto-non-2-enonic acid, an unsaturated derivative of N-acetylneuraminic acid. Warner, T.G., Louie, A., Potier, M., Ribeiro, A. Carbohydr. Res. (1991) [Pubmed]
  2. Most influenza A virus-specific memory cytotoxic T lymphocytes react with antigenic epitopes associated with internal virus determinants. Kees, U., Krammer, P.H. J. Exp. Med. (1984) [Pubmed]
  3. Role of sialic acid in synaptosomal transport of amino acid transmitters. Zaleska, M.M., Erecińska, M. Proc. Natl. Acad. Sci. U.S.A. (1987) [Pubmed]
  4. Lectin activity as a marker for Hodgkin disease cells. Paietta, E., Stockert, R.J., Morell, A.G., Diehl, V., Wiernik, P.H. Proc. Natl. Acad. Sci. U.S.A. (1986) [Pubmed]
  5. Comparison of the structural characteristics of cell-CAM 105 from hepatocytes with those of an altered form expressed by rat transplantable hepatocellular carcinomas. McEntire, K.D., Mowery, J., Hixson, D.C. Cancer Res. (1989) [Pubmed]
  6. Pancreatic islet cell surface glycoproteins containing Gal beta 1-4GlcNAc-R identified by a cytotoxic monoclonal autoantibody. Uchigata, Y., Spitalnik, S.L., Tachiwaki, O., Salata, K.F., Notkins, A.L. J. Exp. Med. (1987) [Pubmed]
  7. A neuraminidase from Trypanosoma cruzi removes sialic acid from the surface of mammalian myocardial and endothelial cells. Libby, P., Alroy, J., Pereira, M.E. J. Clin. Invest. (1986) [Pubmed]
  8. Biosynthesis of a nonphysiological sialic acid in different rat organs, using N-propanoyl-D-hexosamines as precursors. Kayser, H., Zeitler, R., Kannicht, C., Grunow, D., Nuck, R., Reutter, W. J. Biol. Chem. (1992) [Pubmed]
  9. Neutrophil defensins induce histamine secretion from mast cells: mechanisms of action. Befus, A.D., Mowat, C., Gilchrist, M., Hu, J., Solomon, S., Bateman, A. J. Immunol. (1999) [Pubmed]
  10. A mechanism of action for anaphylatoxin C3a stimulation of mast cells. Mousli, M., Hugli, T.E., Landry, Y., Bronner, C. J. Immunol. (1992) [Pubmed]
  11. Glycosphingolipid patterns of the gastrointestinal tract and feces of germ-free and conventional rats. Gustafsson, B.E., Karlsson, K.A., Larson, G., Midtvedt, T., Strömberg, N., Teneberg, S., Thurin, J. J. Biol. Chem. (1986) [Pubmed]
  12. Ependymal denudation, aqueductal obliteration and hydrocephalus after a single injection of neuraminidase into the lateral ventricle of adult rats. Grondona, J.M., Pérez-Martín, M., Cifuentes, M., Pérez, J., Jiménez, A.J., Pérez-Fígares, J.M., Fernández-Llebrez, P. J. Neuropathol. Exp. Neurol. (1996) [Pubmed]
  13. Glycosylation of lactase-phlorizin hydrolase in rat small intestine during development. Büller, H.A., Rings, E.H., Pajkrt, D., Montgomery, R.K., Grand, R.J. Gastroenterology (1990) [Pubmed]
  14. Analysis of the carbohydrate composition of axonally transported glycoconjugates in sciatic nerve. Hart, C.E., Wood, J.G. J. Neurosci. (1986) [Pubmed]
  15. Functional segregation of hepatic receptors for asialoglycoproteins during endocytosis. Stockert, R.J., Howard, D.J., Morell, A.G., Scheinberg, I.H. J. Biol. Chem. (1980) [Pubmed]
  16. The role of cell surface sialic acid in insulin receptor function and insulin action. Hayes, G.R., Lockwood, D.H. J. Biol. Chem. (1986) [Pubmed]
  17. Dual subcellular localization of sialidase in cultured granule cells differentiated in culture. Pitto, M., Giglioni, A., Tettamanti, G. Neurochem. Int. (1992) [Pubmed]
  18. Chronic ethanol increases ganglioside sialidase activity in rat leukocytes, erythrocytes, and brain synaptosomes. Marmillot, P., Rao, M.N., Liu, Q.H., Lakshman, M.R. Alcohol. Clin. Exp. Res. (1999) [Pubmed]
  19. Identification and characterization of a bile acid receptor in isolated liver surface membranes. Accatino, L., Simon, F.R. J. Clin. Invest. (1976) [Pubmed]
  20. Binding of peanut lectin to breast epithelium, human carcinomas, and a cultured rat mammary stem cell: use of the lectin as a marker of mammary differentiation. Newman, R.A., Klein, P.J., Rudland, P.S. J. Natl. Cancer Inst. (1979) [Pubmed]
  21. Atypical familial dysbetalipoproteinemia associated with apolipoprotein phenotype E3/3. Havel, R.J., Kotite, L., Kane, J.P., Tun, P., Bersot, T. J. Clin. Invest. (1983) [Pubmed]
  22. Analysis of cholecystokinin-binding proteins using endo-beta-N-acetylglucosaminidase F. Rosenzweig, S.A., Madison, L.D., Jamieson, J.D. J. Cell Biol. (1984) [Pubmed]
  23. Rapid adhesion of nerve cells to muscle fibers from adult rats is mediated by a sialic acid-binding receptor. Bischoff, R. J. Cell Biol. (1986) [Pubmed]
  24. Changes in the random distribution of sialic acid at the surface of the myeloid sinusoidal endothelium resulting from the presence of diaphragmed fenestrae. De Bruyn, P.P., Michelson, S. J. Cell Biol. (1979) [Pubmed]
  25. Cytoskeletal-membrane interactions: a stable interaction between cell surface glycoconjugates and doublet microtubules of the photoreceptor connecting cilium. Horst, C.J., Forestner, D.M., Besharse, J.C. J. Cell Biol. (1987) [Pubmed]
  26. Biosynthesis and processing of the type II insulin-like growth factor receptor in H-35 hepatoma cells. MacDonald, R.G., Czech, M.P. J. Biol. Chem. (1985) [Pubmed]
  27. Effects of tunicamycin on growth hormone binding in rat adipocytes. Szecowka, J., Tai, L.R., Goodman, H.M. Endocrinology (1990) [Pubmed]
  28. Interaction of purified brush-border membrane aminopeptidase N and dipeptidyl peptidase IV with lectin-sepharose derivatives. Erickson, R.H., Kim, Y.S. Biochim. Biophys. Acta (1983) [Pubmed]
  29. A role for the Mycoplasma pneumoniae adhesin P1 in interleukin (IL)-4 synthesis and release from rodent mast cells. Hoek, K.L., Duffy, L.B., Cassell, G.H., Dai, Y., Atkinson, T.P. Microb. Pathog. (2005) [Pubmed]
  30. Structural analysis and functional role of the carbohydrate component of somatostatin receptors. Rens-Domiano, S., Reisine, T. J. Biol. Chem. (1991) [Pubmed]
  31. Chagas' disease parasite promotes neuron survival and differentiation through TrkA nerve growth factor receptor. Chuenkova, M.V., PereiraPerrin, M. J. Neurochem. (2004) [Pubmed]
  32. Molecular forms of N-CAM and its RNA in developing and denervated skeletal muscle. Covault, J., Merlie, J.P., Goridis, C., Sanes, J.R. J. Cell Biol. (1986) [Pubmed]
  33. Induced and spontaneous neuritogenesis are associated with enhanced expression of ganglioside GM1 in the nuclear membrane. Wu, G., Lu, Z.H., Ledeen, R.W. J. Neurosci. (1995) [Pubmed]
  34. High affinity binding of 125I-angiotensin II to rat glomerular basement membranes. Sraer, J., Baud, L., Cosyns, J.P., Verroust, P., Nivez, M.P., Ardaillou, R. J. Clin. Invest. (1977) [Pubmed]
  35. Human Toxoplasma gondii-specific secretory immunoglobulin A reduces T. gondii infection of enterocytes in vitro. Mack, D.G., McLeod, R. J. Clin. Invest. (1992) [Pubmed]
  36. Peanut agglutinin, a new mitogen that binds to galactosyl sites exposed after neuraminidase treatment. Novogrodsky, A., Lotan, R., Ravid, A., Sharon, N. J. Immunol. (1975) [Pubmed]
  37. A protein with characteristics of factor H is present on rodent platelets and functions as the immune adherence receptor. Alexander, J.J., Hack, B.K., Cunningham, P.N., Quigg, R.J. J. Biol. Chem. (2001) [Pubmed]
  38. Tris inhibits both proteolytic and oligosaccharide processing occurring in the Golgi complex in primary cultured rat hepatocytes. Oda, K., Ogata, S., Koriyama, Y., Yamada, E., Mifune, K., Ikehara, Y. J. Biol. Chem. (1988) [Pubmed]
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