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Gene Review

RBP1  -  retinol binding protein 1, cellular

Homo sapiens

Synonyms: CRABP-I, CRBP, CRBP-I, CRBP1, CRBPI, ...
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Disease relevance of RBP1


High impact information on RBP1


Chemical compound and disease context of RBP1


Biological context of RBP1


Anatomical context of RBP1

  • Traces of CRABP-I mRNA were detected only in the 886 cells, whereas CRABP-II mRNA was detected in the other three cell lines [15].
  • In contrast to CRABP I, CRBP was diffusely present throughout the cervical epithelium irrespective of the state of differentiation or the presence of disease [16].
  • The experimental system consisted of the [3H]retinol-RBP complex, Escherichia coli-expressed recombinant apo-CRBP containing the 10 amino acid long streptavidin-binding peptide sequence at its C terminus (designated as CRBP-Strep) and permeabilized human placental membranes [17].
  • The hypothesis that the cellular uptake of retinol involves the specific interaction of a plasma membrane receptor with serum retinol-binding protein (RBP) at the extracellular surface followed by ligand transfer to cytoplasmic cellular retinol-binding protein (CRBP) has been investigated [17].
  • These observations suggest that CRABP-I and -II are regulated differently in human keratinocytes [18].

Associations of RBP1 with chemical compounds

  • The transfer of retinol from serum retinol-binding protein to cellular retinol-binding protein is mediated by a membrane receptor [17].
  • We show that CRABP-I and -II have similar M(r) values (15,000), but differ in their dissociation constant towards retinoic acid (Kd of 16.6 nM and 50 nM respectively), in pI (4.86 and 5.13) and in their relative mobilities (RF) on PAGE under nondenaturating conditions (RF values 0.65 and 0.44) [18].
  • To bind more efficiently to CRBP, CRABP, or RARs/RXRs, the retinoid may require further metabolic change [19].
  • In tissues, one pathway of retinoic acid synthesis begins with apo-CRBP stimulating retinyl ester hydrolysis by a microsomal, cholate-independent retinyl ester hydrolase to form holo-CRBP [20].
  • Within the cell, two cellular retinoic acid-binding proteins (CRABP-I and -II) and a ROL-binding protein (CRBP-I) regulate the levels of free RA and ROL [21].

Physical interactions of RBP1

  • Apo-CRBP reacted with 4 mM p-(chloromercuri)benzenesulfonic acid lost retinol binding ability but retained the ability to inhibit LRAT, confirming that the inhibition could not be explained by a reduction in the concentration of free retinol.(ABSTRACT TRUNCATED AT 250 WORDS)[22]
  • Epidermoid carcinomas of the oral cavity and oropharynx from six patients were examined for the presence and amount of cellular retinol (CRBP) and cellular retinoic acid-binding (CRABP) proteins [23].
  • Of particular interest, cellular retinol-binding protein 1 (CRBP1) and retinol-binding protein (RBP), two genes whose products are involved in retinol transport and metabolism, were found to be downregulated in this ovarian cancer model system [24].
  • CRBP III was differentiated from purified piscine and rat cellular retinol-binding proteins (CRBP) and cellular retinoic acid-binding proteins (CRABP) by its amino-acid composition, electrophoretic mobility, fluorescence spectra and ligand-binding specificity [25].

Regulatory relationships of RBP1


Other interactions of RBP1


Analytical, diagnostic and therapeutic context of RBP1

  • We assessed the extent of growth inhibition, the stability of the anti-proliferative effect and the mRNA expression levels (by RT-PCR) of RA receptors (RARs), retinoid X receptors alpha (RXR alpha) and cytosolic RA-binding proteins (CRBP I and CRABP II) in treated cells compared with controls [29].
  • CRBP I and CRABP I expression was confirmed by immunohistochemistry [30].
  • METHODS: We evaluated CRBP expression by in situ hybridization in six reduction mammoplasty specimens and 49 human breast carcinoma specimens by use of digoxigenin-labeled RNA probes and in nine cultured mammoplasty specimens by northern or western blot analysis [6].
  • RESULTS: CRBP was expressed in all 15 mammoplasty specimens (normal breast tissue) and in 33 of 35 available specimens of normal tissue adjacent to carcinoma [6].
  • RESULTS: In 35% of ovarian cancer patient samples, there was no detectable CRBP1 expression by real-time PCR [31].


  1. RBP1 family proteins exhibit SUMOylation-dependent transcriptional repression and induce cell growth inhibition reminiscent of senescence. Binda, O., Roy, J.S., Branton, P.E. Mol. Cell. Biol. (2006) [Pubmed]
  2. Studies of cellular retinol-binding protein (CRBP) in squamous-cell carcinomas of the head and neck region. Fex, G., Wahlberg, P., Biörklund, A., Wennerberg, J., Willén, R. Int. J. Cancer (1986) [Pubmed]
  3. Application of retinol to human skin in vivo induces epidermal hyperplasia and cellular retinoid binding proteins characteristic of retinoic acid but without measurable retinoic acid levels or irritation. Kang, S., Duell, E.A., Fisher, G.J., Datta, S.C., Wang, Z.Q., Reddy, A.P., Tavakkol, A., Yi, J.Y., Griffiths, C.E., Elder, J.T. J. Invest. Dermatol. (1995) [Pubmed]
  4. Cellular retinol-binding protein in mucosa, in benign and malignant non-squamous cell tumours of the head and neck. Wahlberg, P., Fex, G., Wennerberg, J., Willén, R. Acta Otolaryngol. (1989) [Pubmed]
  5. A direct repeat in the cellular retinol-binding protein type II gene confers differential regulation by RXR and RAR. Mangelsdorf, D.J., Umesono, K., Kliewer, S.A., Borgmeyer, U., Ong, E.S., Evans, R.M. Cell (1991) [Pubmed]
  6. Cellular retinol-binding protein expression and breast cancer. Kuppumbatti, Y.S., Bleiweiss, I.J., Mandeli, J.P., Waxman, S., Mira-Y-Lopez, R. J. Natl. Cancer Inst. (2000) [Pubmed]
  7. A relationship between retinol and cellular retinol-binding protein concentrations in human squamous cell carcinomas. Wahlberg, P., Fex, G., Wennerberg, J. Biochim. Biophys. Acta (1989) [Pubmed]
  8. DNA methylation of genes linked to retinoid signaling in squamous cell carcinoma of the esophagus: DNA methylation of CRBP1 and TIG1 is associated with tumor stage. Mizuiri, H., Yoshida, K., Toge, T., Oue, N., Aung, P.P., Noguchi, T., Yasui, W. Cancer Sci. (2005) [Pubmed]
  9. Cellular binding proteins for vitamin A in the normal human uterine cervix and in dysplasias. Palan, P.R., Romney, S.L. Cancer Res. (1979) [Pubmed]
  10. Regional mapping of RBP4 to 10q23----q24 and RBP1 to 3q21----q22 in man. Rocchi, M., Covone, A., Romeo, G., Faraonio, R., Colantuoni, V. Somat. Cell Mol. Genet. (1989) [Pubmed]
  11. Identification and structural analysis of a zebrafish apo and holo cellular retinol-binding protein. Calderone, V., Folli, C., Marchesani, A., Berni, R., Zanotti, G. J. Mol. Biol. (2002) [Pubmed]
  12. Fatty acid binding proteins from different tissues show distinct patterns of fatty acid interactions. Richieri, G.V., Ogata, R.T., Zimmerman, A.W., Veerkamp, J.H., Kleinfeld, A.M. Biochemistry (2000) [Pubmed]
  13. Enzymes and binding proteins affecting retinoic acid concentrations. Napoli, J.L., Boerman, M.H., Chai, X., Zhai, Y., Fiorella, P.D. J. Steroid Biochem. Mol. Biol. (1995) [Pubmed]
  14. DNA methylation of genes linked with retinoid signaling in gastric carcinoma: expression of the retinoid acid receptor beta, cellular retinol-binding protein 1, and tazarotene-induced gene 1 genes is associated with DNA methylation. Shutoh, M., Oue, N., Aung, P.P., Noguchi, T., Kuraoka, K., Nakayama, H., Kawahara, K., Yasui, W. Cancer (2005) [Pubmed]
  15. Modulation by retinoic acid (RA) of squamous cell differentiation, cellular RA-binding proteins, and nuclear RA receptors in human head and neck squamous cell carcinoma cell lines. Zou, C.P., Clifford, J.L., Xu, X.C., Sacks, P.G., Chambon, P., Hong, W.K., Lotan, R. Cancer Res. (1994) [Pubmed]
  16. Localization of cellular retinoid-binding proteins in human cervical intraepithelial neoplasia and invasive carcinoma. Hillemanns, P., Tannous-Khuri, L., Koulos, J.P., Talmage, D., Wright, T.C. Am. J. Pathol. (1992) [Pubmed]
  17. The transfer of retinol from serum retinol-binding protein to cellular retinol-binding protein is mediated by a membrane receptor. Sundaram, M., Sivaprasadarao, A., DeSousa, M.M., Findlay, J.B. J. Biol. Chem. (1998) [Pubmed]
  18. Expression of CRABP-I and -II in human epidermal cells. Alteration of relative protein amounts is linked to the state of differentiation. Siegenthaler, G., Tomatis, I., Chatellard-Gruaz, D., Jaconi, S., Eriksson, U., Saurat, J.H. Biochem. J. (1992) [Pubmed]
  19. N-(4-hydroxyphenyl)retinamide: interactions with retinoid-binding proteins/receptors. Sani, B.P., Shealy, Y.F., Hill, D.L. Carcinogenesis (1995) [Pubmed]
  20. Physiological occurrence, biosynthesis and metabolism of retinoic acid: evidence for roles of cellular retinol-binding protein (CRBP) and cellular retinoic acid-binding protein (CRABP) in the pathway of retinoic acid homeostasis. Napoli, J.L., Posch, K.P., Fiorella, P.D., Boerman, M.H. Biomed. Pharmacother. (1991) [Pubmed]
  21. Extra-and intracellular transport of retinoids: a reappraisal. Siegenthaler, G. Horm. Res. (1996) [Pubmed]
  22. Differential interaction of lecithin-retinol acyltransferase with cellular retinol binding proteins. Herr, F.M., Ong, D.E. Biochemistry (1992) [Pubmed]
  23. Presence of cellular retinol and retinoic acid-binding proteins in epidermoid carcinoma of the oral cavity and oropharynx. Ong, D.E., Goodwin, W.J., Jesse, R.H., Griffin, A.C. Cancer (1982) [Pubmed]
  24. Decreased expression of retinol-binding proteins is associated with malignant transformation of the ovarian surface epithelium. Roberts, D., Williams, S.J., Cvetkovic, D., Weinstein, J.K., Godwin, A.K., Johnson, S.W., Hamilton, T.C. DNA Cell Biol. (2002) [Pubmed]
  25. Purification and partial characterization of a novel cellular retinol-binding protein, type three, from the piscine eyes. Nishiwaki, S., Kato, M., Okuno, M., Kanai, M., Muto, Y. Biochim. Biophys. Acta (1990) [Pubmed]
  26. Cellular retinol-binding proteins are determinants of retinol uptake and metabolism in stably transfected Caco-2 cells. Levin, M.S. J. Biol. Chem. (1993) [Pubmed]
  27. Binding of a member of the NF1 family of transcription factors to two distinct cis-acting elements in the promoter and 5'-flanking region of the human cellular retinol binding protein 1 gene. Eskild, W., Simard, J., Hansson, V., Guérin, S.L. Mol. Endocrinol. (1994) [Pubmed]
  28. The retinal pigment epithelium: a versatile partner in vision. Bok, D. J. Cell Sci. Suppl. (1993) [Pubmed]
  29. All-trans, 13-cis and 9-cis retinoic acids induce a fully reversible growth inhibition in HNSCC cell lines: implications for in vivo retinoic acid use. Giannini, F., Maestro, R., Vukosavljevic, T., Pomponi, F., Boiocchi, M. Int. J. Cancer (1997) [Pubmed]
  30. Biosynthesis and function of all-trans- and 9-cis-retinoic acid in parathyroid cells. Liu, W., Hellman, P., Li, Q., Yu, W.R., Juhlin, C., Nordlinder, H., Rollman, O., Akerström, G., Törmä, H., Melhus, H. Biochem. Biophys. Res. Commun. (1996) [Pubmed]
  31. Loss of cellular retinol-binding protein 1 gene expression in microdissected human ovarian cancer. Cvetković, D., Williams, S.J., Hamilton, T.C. Clin. Cancer Res. (2003) [Pubmed]
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