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Gene Review

Mmp3  -  matrix metallopeptidase 3

Rattus norvegicus

Synonyms: MMP-3, Matrix metalloproteinase-3, PTR1 protein, SL-1, Stromelysin-1, ...
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Disease relevance of Mmp3


High impact information on Mmp3

  • We report here that nerve growth factor (NGF) induced the gene encoding the metalloprotease transin in PC12 cells with a time course coincident with the initial appearance of neurites by these cells [5].
  • Acidic and basic fibroblast growth factors also stimulated transin mRNA expression and neurite outgrowth, whereas various other agents had no effects on either of these phenomena [5].
  • Finally, we show that sequences contained within 750 bp of the 5' untranscribed region of the transin gene confer responsiveness to NGF and dexamethasone [5].
  • Rat hepatic lipocytes synthesize and secrete transin (stromelysin) in early primary culture [6].
  • However, induction of delayed NGF response genes such as TGF-beta1 and MMP-3 was inhibited [7].

Chemical compound and disease context of Mmp3


Biological context of Mmp3


Anatomical context of Mmp3

  • Moreover, the levels of MMP-3 were also found to be increased significantly in the rat PD brain model produced by the cerebral injection of 6-hydroxydopamine into the substantia nigra [12].
  • Double labeling with GFAP confirmed the presence of MMP-3 within reactive astrocytes induced by each injury model [13].
  • MMP-3 co-localized with activated microglia (Ox-42+) and ischemic neurons (NeuN+) [14].
  • MMP-3 in microglia/macrophages may be activating proMMP-9 [14].
  • The expression of MMP-9 and MMP-3 was localized mainly to the cerebrovasculature in LPS-stimulated brain tissue, predominantly in the perivascular cells of the basal lamina near the site of injection [1].

Associations of Mmp3 with chemical compounds

  • Dibutyryl cyclic AMP also induced transin RNA [10].
  • Whereas the calcium ionophore A23187 and the tumor promoter TPA, either alone or administered together, did not increase transin RNA levels, TPA could synergise with a serum factor to effect such an increase [10].
  • Incubation in 10(-7) and 10(-5) M fluoride resulted in a decrease in expression of the 45-kD MMP, sharp increases in the expression of MMP-3, and the appearance of a band at 110 kD, which showed immunoreactivity for MMP-9 [15].
  • Preliminary characterization of this staurosporine-sensitive kinase suggest that it does not correspond to a tyrosine kinase, nor various serine kinases, and that it is involved both at the transcriptional and posttranscriptional levels of transin gene regulation [16].
  • We show that a synthetic peptide PRCGVPDV is capable of acting as a weak inhibitor of transin and that replacement of the Cys with a Ser abolishes inhibition by the peptide [17].

Regulatory relationships of Mmp3


Other interactions of Mmp3


Analytical, diagnostic and therapeutic context of Mmp3

  • Semi-quantitative RT-PCR revealed that MMP-3 mRNA also increased after each injury, however, the combined insult induced a much greater elevation than fluid percussion alone: 1.9-fold vs. 79%, respectively [13].
  • Immunofluorescence staining of sections of rat mammary gland with antibodies to 72 KD gelatinase (MMP-2) and stromelysin (MMP-3) revealed increased production of these two proteinases during involution [25].
  • The expression of MMP-1 and MMP-3 increased 3-fold (p < 0.001) within the renal cortex 24 h following nephrectomy, and then gradually decreased to the control level after 7 days [26].
  • In the present study we observed that human Schwann cells and cutaneous neurofibroma Schwann cell cultures secreted abundant MMP-3 and their proliferation was inhibited by autologous and rat Schwann cell conditioned media [27].
  • Transin messenger RNA in lipocytes was analyzed by Northern blotting [6].


  1. Stromelysin-1 and gelatinase A are upregulated before TNF-alpha in LPS-stimulated neuroinflammation. Mun-Bryce, S., Lukes, A., Wallace, J., Lukes-Marx, M., Rosenberg, G.A. Brain Res. (2002) [Pubmed]
  2. Purification and properties of extracellular matrix-degrading metallo-proteinase overproduced by Rous sarcoma virus-transformed rat liver cell line, and its identification as transin. Umenishi, F., Yasumitsu, H., Ashida, Y., Yamauti, J., Umeda, M., Miyazaki, K. J. Biochem. (1990) [Pubmed]
  3. Spatial and temporal pattern of expression of interstitial collagenase, stromelysin/transin, gelatinase A, and TIMP-1 during experimental gastric ulcer healing. Calabrò, A., Grappone, C., Pellegrini, G., Evangelista, S., Tramontana, M., Schuppan, D., Herbst, H., Milani, S. Digestion (2004) [Pubmed]
  4. Differential regulation of matrix metalloproteinase-3 gene expression in endometriotic lesions compared with endometrium. Cox, K.E., Piva, M., Sharpe-Timms, K.L. Biol. Reprod. (2001) [Pubmed]
  5. NGF induction of the gene encoding the protease transin accompanies neuronal differentiation in PC12 cells. Machida, C.M., Rodland, K.D., Matrisian, L., Magun, B.E., Ciment, G. Neuron (1989) [Pubmed]
  6. Rat hepatic lipocytes synthesize and secrete transin (stromelysin) in early primary culture. Vyas, S.K., Leyland, H., Gentry, J., Arthur, M.J. Gastroenterology (1995) [Pubmed]
  7. The transcriptional corepressor NAB2 inhibits NGF-induced differentiation of PC12 cells. Qu, Z., Wolfraim, L.A., Svaren, J., Ehrengruber, M.U., Davidson, N., Milbrandt, J. J. Cell Biol. (1998) [Pubmed]
  8. Expression of the transin, c-fos, and c-jun genes in rat transplantable osteosarcomas and malignant fibrous histiocytomas. Honoki, K., Tsutsumi, M., Tsujiuchi, T., Kondoh, S., Shiraiwa, K., Miyauchi, Y., Mii, Y., Tamai, S., Konishi, Y., Bowden, G.T. Mol. Carcinog. (1992) [Pubmed]
  9. Transcription factor Ets-1 is essential for mesangial matrix remodeling. Mizui, M., Isaka, Y., Takabatake, Y., Sato, Y., Kawachi, H., Shimizu, F., Takahara, S., Ito, T., Imai, E. Kidney Int. (2006) [Pubmed]
  10. Isolation of the oncogene and epidermal growth factor-induced transin gene: complex control in rat fibroblasts. Matrisian, L.M., Leroy, P., Ruhlmann, C., Gesnel, M.C., Breathnach, R. Mol. Cell. Biol. (1986) [Pubmed]
  11. Peptide YY and neuropeptide Y induce villin expression, reduce adhesion, and enhance migration in small intestinal cells through the regulation of CD63, matrix metalloproteinase-3, and Cdc42 activity. Lee, M., Hadi, M., Halldén, G., Aponte, G.W. J. Biol. Chem. (2005) [Pubmed]
  12. Proteolytic cleavage of extracellular secreted {alpha}-synuclein via matrix metalloproteinases. Sung, J.Y., Park, S.M., Lee, C.H., Um, J.W., Lee, H.J., Kim, J., Oh, Y.J., Lee, S.T., Paik, S.R., Chung, K.C. J. Biol. Chem. (2005) [Pubmed]
  13. Elevation of hippocampal MMP-3 expression and activity during trauma-induced synaptogenesis. Kim, H.J., Fillmore, H.L., Reeves, T.M., Phillips, L.L. Exp. Neurol. (2005) [Pubmed]
  14. Immunohistochemistry of matrix metalloproteinases in reperfusion injury to rat brain: activation of MMP-9 linked to stromelysin-1 and microglia in cell cultures. Rosenberg, G.A., Cunningham, L.A., Wallace, J., Alexander, S., Estrada, E.Y., Grossetete, M., Razhagi, A., Miller, K., Gearing, A. Brain Res. (2001) [Pubmed]
  15. Altered expression of matrix metalloproteinases within mineralizing bone cells in vitro in the presence of fluoride. Waddington, R.J., Langley, M.S. Connect. Tissue Res. (2003) [Pubmed]
  16. NGF-induction of the metalloproteinase-transin/stromelysin in PC12 cells: involvement of multiple protein kinases. Machida, C.M., Scott, J.D., Ciment, G. J. Cell Biol. (1991) [Pubmed]
  17. Mutational analysis of the transin (rat stromelysin) autoinhibitor region demonstrates a role for residues surrounding the "cysteine switch". Park, A.J., Matrisian, L.M., Kells, A.F., Pearson, R., Yuan, Z.Y., Navre, M. J. Biol. Chem. (1991) [Pubmed]
  18. Cytokine interactions promote synergistic fibronectin accumulation by mesangial cells. Pawluczyk, I.Z., Harris, K.P. Kidney Int. (1998) [Pubmed]
  19. Activation of matrix metalloproteinase-3 and agrin cleavage in cerebral ischemia/reperfusion. Solé, S., Petegnief, V., Gorina, R., Chamorro, A., Planas, A.M. J. Neuropathol. Exp. Neurol. (2004) [Pubmed]
  20. Heparin selectively inhibits gene expression of matrix metalloproteinase transin in cultured mesangial cells. Kitamura, M., Maruyama, N., Mitarai, T., Nagasawa, R., Yokoo, T., Sakai, O. Biochem. Biophys. Res. Commun. (1994) [Pubmed]
  21. Regulation of Peripheral Inflammation by Spinal p38 MAP Kinase in Rats. Boyle, D.L., Jones, T.L., Hammaker, D., Svensson, C.I., Rosengren, S., Albani, S., Sorkin, L., Firestein, G.S. PLoS Med. (2006) [Pubmed]
  22. Dynamic changes in the expression of matrix metalloproteinases and their inhibitors, TIMPs, during hepatic fibrosis induced by alcohol in rats. Xu, G.F., Li, P.T., Wang, X.Y., Jia, X., Tian, D.L., Jiang, L.D., Yang, J.X. World J. Gastroenterol. (2004) [Pubmed]
  23. Spatiotemporal expression patterns of metalloproteinases and their inhibitors in the postnatal developing rat cerebellum. Vaillant, C., Didier-Bazès, M., Hutter, A., Belin, M.F., Thomasset, N. J. Neurosci. (1999) [Pubmed]
  24. The first stage of transforming growth factor beta1 activation is release of the large latent complex from the extracellular matrix of growth plate chondrocytes by matrix vesicle stromelysin-1 (MMP-3). Maeda, S., Dean, D.D., Gomez, R., Schwartz, Z., Boyan, B.D. Calcif. Tissue Int. (2002) [Pubmed]
  25. Enhanced synthesis of gelatinase and stromelysin by myoepithelial cells during involution of the rat mammary gland. Dickson, S.R., Warburton, M.J. J. Histochem. Cytochem. (1992) [Pubmed]
  26. Effect of unilateral nephrectomy on gene expression of metalloproteinases and their inhibitors. Koide, H., Nakamura, T., Ebihara, I., Tomino, Y. Nephron (1997) [Pubmed]
  27. Differences in proliferation and invasion by normal, transformed and NF1 Schwann cell cultures are influenced by matrix metalloproteinase expression. Muir, D. Clin. Exp. Metastasis (1995) [Pubmed]
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