Disease relevance of EPHA1

• This protein, hepatoma transmembrane kinase or Htk, shares amino acid similarity with the EPH subfamily of tyrosine kinases [1].
• Insofar as possible, our definition of pre-eclampsia corresponds with EPH in the descriptive classification of the Organisation Gestosis and to 'severe pre-eclampsia' in Nelson's classification [2].
• This retrospective clinical review of reports by several authors of 253 children with extrahepatic (EPH) and 87 children with intrahepatic (IPH) portal hypertension who have undergone operations for variceal hemorrhage presents the long-term follow-up based on the type of management provided [3].
• Edema, proteinuria, hypertension (EPH) gestosis is accompanied by an increase of collagen content and premature replacement of hyaluronic acid by sulfated glycosaminoglycans both in the umbilical cord arteries and in Wharton's jelly [4].
• OBJECTIVE: Edema, proteinuria, hypertension (EPH) gestosis, also known as preeclampsia, is the most common, pregnancy-associated pathological syndrome [5].

High impact information on EPHA1

• Ligands for EPH-related receptor tyrosine kinases that require membrane attachment or clustering for activity [6].
• The EPH-like receptor tyrosine kinase (RTK) HEK (human EPH-like kinase) was identified previously as a membrane antigen on the LK63 human pre-B-cell line and overexpression in leukemic specimens and cell lines suggested a role in oncogenesis [7].
• HEK is a member of the EPH-like receptor tyrosine kinase family, which appear to have roles in development and oncogenesis [8].
• The knowledge about intracellular pathways of signal transduction mediated by EPH-related receptors is still limited [9].
• cDNA cloning and tissue distribution of five human EPH-like receptor protein-tyrosine kinases [10].

Chemical compound and disease context of EPHA1

• The taurine concentration and uptake in platelets obtained from normal pregnant women and gestosis patients with edema, proteinuria and hypertension (EPH gestosis) were investigated [11].
• HPL and estriol serum levels of placental perfusion as indexes of placental function in EPH gestosis and placental insufficiency [12].
• Since prostacyclins lower arterial resistance yet at the same time are strong inhibitors of thrombocyte aggregation, this prostacyclin deficiency could account for the hypertension and the occurrence of platelet thrombi in the placental bloodstream associated with EPH gestosis [13].
• We also analyzed the relationship between these conditions and the type and severity of hypertension, gestational age, presence of symptoms of the classic EPH triad and of abnormal uric acid values, hemoconcentration, and low urinary estriol values [14].
• Primary aldosteronism caused by aldosterone-producing adenoma in pregnancy--complicated by EPH gestosis [15].

Biological context of EPHA1

• To permit mutational analysis, the complete genomic structure of the human EPHA1 gene on chromosome 7q34 was determined and oligonucleotide pairs were designed to amplify coding regions [16].
• Presumed sequencing errors in the published cDNA sequence of EPHA1 were identified in exons 10 and 11 [16].
• Ephrin/EPH signaling pathway networks with the WNT signaling pathway during embryogenesis, tissue regeneration, and carcinogenesis [17].
• TCF/LEF-binding sites within the promoter region of human EPH family members were searched for by using bioinformatics and human intelligence [17].
• In this study, plasma levels of magnesium, calcium, zinc and copper were simultaneously determined in pregnancies complicated by either abortion, intrauterine growth retardation (IUGR), diabetes or EPH (edema, proteinuria, hypertension) gestosis [18].

Anatomical context of EPHA1

• Shunt operations are preferred over direct operative procedures in patients with EPH when mesenteric veins suitable for shunting are available [3].
• Studies were performed on normal umbilical cord arteries and those taken from newborns of mothers with EPH gestosis [4].
• METHODS: Contact-inhibited human fibroblasts were incubated in Dulbecco culture medium containing control or EPH umbilical cord serum and supplemented with (14)C-glucosamine or (35)S-sulfate [5].
• ET concentration in umbilical venous blood serum in 22 cases of EPH gestosis (group Gp) and 20 cases from the control group (Kp) was measured after delivery [19].
• Different isoenzyme A patterns in homogenates were observed in placentas obtained from normal and prolonged pregnancies and in those complicated by EPH gestosis [20].

Associations of EPHA1 with chemical compounds

• Genomic structure of the EPHA1 receptor tyrosine kinase gene [16].
• EPH serum was much more active in stimulation of sulfated GAGs biosynthesis than control serum, whereas the biosynthesis of hyaluronic acid was stimulated by both sera to a similar degree [5].
• A thermal gradient (temperature range 7-45 degrees C) was used to assess ambient temperature (Ta) preferences of rats following treatment with clonidine (25 microg/kg), norepinephrine (NE, 250 microg/kg), isoproterenol (ISO, 50 microg/kg), and ephedrine (EPH, 10 mg/kg) [21].
• In contrast to the response of non-pregnant women, healthy pregnant women and women with EPH gestosis showed a significantly smaller increase in ANF secretion induced by WI [22].
• The present study was undertaken in order to determine the changes in blood levels of lipid peroxide and vitamin E in EPH gestosis [23].

Other interactions of EPHA1

• Ligands of EPH family receptors are called ephrins, which include ephrin-A and ephrin-B subgroups [24].
• BACKGROUND: The EPH family is the largest subfamily of receptor protein-tyrosine kinases, consisting of EPHA and EPHB subgroups [24].
• ECK, a human EPH-related gene, maps to 1p36.1, a common region of alteration in human cancers [25].
• According to Kaplan-Meier estimates, high EPHA1, and low SMAD1 and MARCO expression were associated with better progression-free survival (PFS) [26].

Analytical, diagnostic and therapeutic context of EPHA1

• Molecular cloning of a ligand for the EPH-related receptor protein-tyrosine kinase Htk [27].
• Our findings demonstrate the application of biosensor technology in ligand purification and show that AL-1, as has been found for other ligands of the EPH-like RTK family, binds more than one receptor [7].
• In this study we have used a more directed approach to identify new members of the EPH/elk-family by PCR of human embryonic cDNA: we utilized oligonucleotide primers specifically designed to a highly conserved N-terminal motif and the kinase region of EPH/elk-PTKs in RNA-PCRs [28].
• For these mothers, the incidence of EPH gestosis on admission was 42.5%, in contrast to 31.4% for a control group matched for age, parity and nationality [29].
• It is concluded that dependence on overeating in pregnancy may result in higher rates of EPH gestosis, cesarean section and perinatal mortality [30].

References