Disease relevance of TES

• Mutation analysis of the coding TES exons in 21 human tumour-derived cell lines revealed the presence of a frameshift mutation in one allele in the breast cancer cell line ZR-75 [1].
• Methylation of the CpG island at the 5' end of TES appears to be a remarkably frequent finding, occurring in seven out of 10 ovarian carcinomas and in each of the 30 tumour-derived cell lines tested [1].
• Although TESTIN mRNA is expressed in all normal human tissues examined, we observed lack of expression in 22% of cancer cell lines and 44% of the cell lines derived from hematological malignancies [2].
• Downregulation of RUNX3 and TES by hypermethylation in glioblastoma [3].
• 3. Such predicted minimal TIS and TES were compared with observed minimal values from five healthy subjects who tried to reach their maximal ventilations during three experimental conditions: maximal voluntary hyperventilation, rebreathing, and graded exercise [4].

Psychiatry related information on TES

• CONCLUSIONS: The results of this study call into question the use of the TES and the interpretation of a large body of literature purporting to study the relationship of teachers' self-efficacy beliefs to important educational outcomes [5].
• The Toxic Exposure Surveillance System (TESS) of the American Association of Poison Control Systems offers an opportunity to evaluate the epidemiology of volatile substance abuse using a data set that captures data from a large geographic area covering a wide-ranging group of socioeconomic strata, ethnic groups, and demographics [6].

High impact information on TES

• Metal-ion affinity (formation) constants were determined for two "Good's" buffers, N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid (TES) and N,N-bis(2-hydroxyethyl)glycine (bicine) [7].
• The metal chelates formed undergo loss of an internal ligand (alcohol) proton (bicine) and undergo hydrolysis (bicine and TES) and dimerization reactions (TES) [7].
• Estimates of cytotoxicity obtained using the lymphocyte titration assay were reproducible upon repeated testin over the course of several months and were relatively unaffected by two- to fourfold variations in target cell concentration [8].
• Thus, testin can become tightly associated with Sertoli cell membrane upon its secretion when intercellular junctions are formed [9].
• In the in vitro studies using Sertoli cells cultured at high cell density, where specialized junctions were being formed, the concentration of "soluble" testin in the spent media was greatly reduced versus monolayer cultures at low cell density, where specialized junctions were absent [9].

Chemical compound and disease context of TES

• Oxidized Fe protein from Azotobacter vinelandii (Av2(0)) was reduced by dithionite (DT) in the absence and presence of nucleotides, over the temperature range 10-40 degrees C, over the pH range 7-8, and in various buffers--inorganic phosphate, TES, HEPES, and Tris [10].
• We examined by TES-enzyme-linked immunosorbent test and immunoblot assay for toxocarosis and cysticercosis the baseline and final follow-up sera of 10 pigs, before and after (3 months) infection with Taenia solium [11].
• With HCO3 solution, anoxic H1 efflux rate was approximately 50% of normoxia (0.333 vs. 0.645 mmol.l-1.min-1), but in TES solution, H1 efflux rate was unaffected by anoxia [12].

Biological context of TES

• Previously, we identified TES as a novel candidate tumour suppressor gene that mapped to human chromosome 7q31 [13].
• The fact that TES localises to regions of cell adhesion suggests that it functions in events related to cell motility and adhesion [13].
• The TES gene lies within the minimal region of overlap of several LOH studies and appears to possess the properties of a tumour suppressor [1].
• The TES gene at 7q31.1 is methylated in tumours and encodes a novel growth-suppressing LIM domain protein [1].
• Extensive analysis of the 7q31 region in human prostate tumors supports TES as the best candidate tumor suppressor gene [14].

Anatomical context of TES

• TES is a novel focal adhesion protein with a role in cell spreading [13].
• In support of this, we demonstrate that fibroblasts stably overexpressing TES have an increased ability to spread on fibronectin [13].
• RNAi knockdown of the focal adhesion protein TES reveals its role in actin stress fibre organisation [15].
• Together with TES's interacting partners, these data suggest that TES might be involved in regulation of the actin cytoskeleton [15].
• As well as localising to cell-matrix adhesions, TES localises to cell-cell contacts and to actin stress fibres [15].

Associations of TES with chemical compounds

• Bicine and TES buffer not only hydrogen ions but also metal ions [7].
• Under these conditions, conductance increased from 7.0 pS at 22 degrees C to 10.9 pS at 37 degrees C. The conductance at 22 degrees C was approximately 1.0 pS higher when TES and HEPES were omitted from the solution, suggesting weak, voltage-independent block by pH buffers [16].
• The final separation buffer consisted of 80 mM sodium cholate in 50 mM N-tris (hydroxymethyl)methyl-2-aminoethanesulfonic acid (TES), pH 7 [17].
• [reaction: see text] Addition of magnesium anion 4 (M = MgBr, R(1) = TES) to ketone 6 (R(2) = PMB) at -78 degrees C in THF proceeded under chelation control to provide alcohol 7, which possesses the full set of the chiral centers of fostriecin [18].
• [reaction: see text] A triphenylmethyl (trityl) ether was reductively and instantaneously cleaved by triethylsilane in the presence of a catalytic amount of TES- (or TMS)-triflate [19].

Regulatory relationships of TES

• We believe that TES is a tumour suppressor gene that is inactivated primarily by transcriptional silencing resulting from CpG island methylation [1].

Other interactions of TES

• We found that CAV1, CAV2, MET and TES mRNA expression was lower in prostate tumors than in normal prostate tissues [14].
• Accordingly, most of TES- and Poa p IX-specific T cell clones derived from cultures containing IFN-gamma or IFN-alpha displayed strong cytolytic activity [20].
• After LPS1, TNF-alpha AUC was greater in TES than CON (P<0.05) [21].
• Three patients also underwent transcranial bifocal electric cortical stimulation (TES) and MEPs were recorded from the APB muscle, and CMCT, MEP threshold and amplitude were measured [22].
• DESIGN: Semen aliquots from normozoospermic donors either were subjected to conventional semen freezing (TES and Tris yolk buffer in 7.4% final glycerol) with post-thaw processing or were preprocessed and frozen in HEPES-buffered human tubal fluid with 1% human serum albumin, 4% sucrose, and 6% glycerol [23].

Analytical, diagnostic and therapeutic context of TES

• The specificity of the recombinant Toxocara canis antigen developed for the immunodiagnosis of human toxocariasis was compared with that of the excretory-secretory antigen from T. canis second-stage larvae (TES) by enzyme-linked immunosorbent assay [24].
• METHODS: From September 1995 to January 1998, 24 patients (11 men and 13 women; mean age, 28.2 years) underwent repeat transthoracic sympathectomy (TES) [25].
• Patients' assessments of pain intensity and pain relief suggested better analgesia with buprenorphine TES than with placebo, although the differences did not reach statistical significance [26].
• The repeat TES was performed with patients under general anesthesia using either a standard single-lumen endotracheal tube (12 patients) or a double-lumen endotracheal tube (12 patients) [25].
• TES and Tris yolk buffer was most effective at protecting sperm from the negative effects of the cryopreservation process [27].

References