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Gene Review

LOC287004  -  Mg1 protein

Rattus norvegicus

Synonyms: GB.7, Pinc
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Disease relevance of LOC287004


High impact information on LOC287004


Chemical compound and disease context of LOC287004

  • 2. [125I]-[Sar1,Ile2]AII binding capacity was increased in lung membranes from rats exposed to hypoxia (10% fractional inspired O2) for 7 days compared to normal rats (Bmax 108 +/- 12 vs 77 +/- 3 fmol mg-1 protein; P < 0.05), with no significant change in dissociation constant [10].
  • Agmatine, detected chemically and immunocytochemically, is contained in cultured astrocytes and C6 glioma cells (8.5 +/- 1.4 and 1.8 +/- 0.6 nmol mg-1 protein, respectively) [11].

Biological context of LOC287004

  • High-affinity [3H]nitrendipine binding in guinea pig synaptosomes (KD = 1.2 X 10(-10) M, Bmax = 0.56 pmol mg-1 protein) was competitively displaced with high affinity (IC50 2.3 X 10(-9) M) by BAY K 8644 [12].
  • When [3H]-Ap4A was used for binding studies a Kd value of 0.10 +/- 0.014 nM and a Bmax value of 16.6 +/- 1.2 fmol mg-1 protein were obtained for the high affinity binding site from the Scatchard analysis [13].
  • Binding capacity (Bmax) for (-)-[3H]DHA increased progressively from 46 +/- 7 on day 18 of gestation to 510 +/- 70 femtomoles . mg-1 protein (mean +/- S.D.) on postnatal day 28, at which time adult Bmax was attained [14].
  • The addition of 5 mM L-DOPA to the incubation medium reduced by 30% (P < 0.02) the maximal rate of decarboxylation of L-5-HTP (Vmax = 56.7 +/- 3.1 nmol mg-1 protein h-1, n = 10) and resulted in a significant (P < 0.05) increase in Km values (249 [228, 270] microM, n = 10) [15].
  • A substrate stereoselectivity was observed in that the turnover number for the methylation of the S-(-)-isomer was 0.25 pmol mg-1 protein h-1, whereas that for the R-(+)-isomer was 2.11 [16].

Anatomical context of LOC287004

  • This effect was species-specific as similar findings were obtained with hippocampal membranes (Bmax 1430 +/- 111 fmol mg-1 protein in gerbil, compared to 196 +/- 31 in rat) [17].
  • 3. Radioligand binding studies performed on a crude granule cell membrane fraction indicated the presence of an apparently homogeneous population of stereo-specific Ins(1,4,5)P3 receptors (KD 54.7 +/- 2.0 nM; Bmax 1.37 +/- 0.29 pmol mg-1 protein) [18].
  • Saturation data indicate that a single population of binding sites exist for [3H]-yohimbine in the cortex (Bmax 121 +/- 10 fmol mg-1, protein; Kd 5.2 +/- 0.9 nM) and hippocampus (Bmax 72 +/- 6 fmol mg-1 protein; Kd 5.8 +/- 0.7 nM) [19].
  • 4. In the rat spinal cord [3H]-MK 912 bound to alpha 2A- and alpha 2C-adrenoceptor sites with similar affinities as in the cerebral cortex and with densities 172 and 7.4 fmol mg-1 protein, respectively [20].
  • The maximal number of binding sites (Bmax) ranged from 5.1 +/- 0.48 to 12 +/- 1.8 pmol mg-1 protein in left atrium and left ventricle, respectively [21].

Associations of LOC287004 with chemical compounds

  • 3. (-)-Isoprenaline infusion (400 micrograms kg-1 h-1) for 14 days caused no significant change in the density of (-)-[125I]-CYP binding which was 48.9 +/- 12.8 and 40.6 +/- 12.3 fmol mg-1 protein in control and isoprenaline-treated animals respectively (n = 6) (P = 0.97) [22].
  • 2. Bay K 8644 (200 microM) reduced State 3 respiration from 247.2 +/- 11.7 to 174.4 +/- 0.06 ng atoms O2 min-1 mg-1 protein, reduced the respiratory control index (RCI) from 5.3 +/- 0.45 to 1.1 +/- 0.03 and reduced the ADP:O ratio from 2.75 +/- 0.03 to 1.3 +/- 0.15 [23].
  • 4. Forskolin, 10 microM, which stimulates both soluble and particulate adenylyl cyclase, maximally relaxed rat aorta and increased cyclic AMP levels from 15 to 379 pmol mg-1 protein at 15 min, but did not significantly relax or increase cyclic AMP levels in human umbilical artery [24].
  • In C6delta cells the level of basal [35S]-GTPgammaS binding was reduced by 51.9+/-6.1 fmols mg-1 protein, whereas in C6mu; and C6 wild-type cells treatment with PTX reduced basal [35S]-GTPgammaS binding by only 10.0+/-3.5 and 8.6+/-3.1 fmols mg-1 protein respectively [25].
  • Saturation analysis revealed the presence of specific glutamate-sensitive DL-[3H]-APB binding sites, with a KD = 1.26 microM and Bmax = 12.08 pmol mg-1 protein [26].

Other interactions of LOC287004

  • 2. Saturation binding experiments with [125I]-PD151242 revealed high affinity binding to a single population of ETA receptors in the cerebellar homogenates (pKd = 9.95 +/- 0.14; Bmax = 30 +/- 15 fmol mg-1 protein) [27].
  • In control rats, isoproterenol administration caused significant depletion of myocardial SOD (1.7 +/- 0.2 units mg-1 protein) and GPx (104 +/- 2mU mg-1 protein) activities and increase in GSH (551.7 +/- 30.9, microg g-1 wet weight of tissue) level, with evidence of myocardial necrosis [28].
  • The specific binding of a single concentration of GR65630 (0.5 nM) defined by granisetron (10 microM) in these areas indicated that the density of 5-HT3 recognition sites varied from 2.4 +/- 1.0 to 10.1 +/- 1.0 fmol mg-1 protein [29].
  • The 5-HT2 receptor agonist alpha-methyl-5-HT (100 to 3000 nM) did not induce any significant changes in Na+, K(+)-ATPase activity (5.0 +/- 1.5 mumol Pi mg-1 protein h-1; n = 4) [30].
  • Increased basal myocardial antioxidant SOD (9.3 +/- 1.2 vs 3.7 +/- 0.7 units mg-1 protein; P<0.05) and catalase activities (34.3 +/- 5.4 vs 17.9 +/- 5.1 units mg-1 protein; P< 0.05) were observed in the Os 50 group only without any evidence of cellular injury in both the groups [28].

Analytical, diagnostic and therapeutic context of LOC287004

  • In contrast, a single binding component with a Kd value of 24 nM and an apparent Bmax value of 0.74 pmol mg-1 protein was observed with a filtration assay [31].
  • 3. Using a centrifugation assay, saturation experiments revealed two distinct binding components with Kd values of 9 and 200 nM, and corresponding Bmax values of 0.55 and 1.00 pmol mg-1 protein [31].
  • Thromboxane B2 synthesis in glomeruli isolated from rats with bile duct ligation was also significantly higher than in sham-operated rats: 12.6 +/- 2.0 versus 6.4 +/- 0.9 pmol h-1 mg-1 protein (P < 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)[32]
  • Exposure of cell cultures to glucagon (10(-7) mol l-1) for only 10 min raised cellular cAMP levels to > 200 pmol mg-1 protein, and suppressed VLDL secretion during the next 24 h to < 40% of control [33].
  • The mean cAMP content of the aortas, measured by radioimmunoassay, in groups C, D E and G was 439, 546, 681, and 1394 mumol mg-1 protein, respectively [34].


  1. Pulmonary artery NADPH-oxidase is activated in hypoxic pulmonary vasoconstriction. Marshall, C., Mamary, A.J., Verhoeven, A.J., Marshall, B.E. Am. J. Respir. Cell Mol. Biol. (1996) [Pubmed]
  2. Cardioprotective effects of N-hydroxyguanidine PR5 in myocardial ischaemia and reperfusion in rats. Veveris, M., Dambrova, M., Cirule, H., Meirena, D., Kalvinsh, I., Wikberg, J.E. Br. J. Pharmacol. (1999) [Pubmed]
  3. A comparison of the binding characteristics of recombinant P2X1 and P2X2 purinoceptors. Michel, A.D., Lundström, K., Buell, G.N., Surprenant, A., Valera, S., Humphrey, P.P. Br. J. Pharmacol. (1996) [Pubmed]
  4. Somatostatin binding in normal and malignant human gastrointestinal mucosa. Miller, G.V., Farmery, S.M., Woodhouse, L.F., Primrose, J.N. Br. J. Cancer (1992) [Pubmed]
  5. Membrane proteins of the myocytes in cardiac overload. Mansier, P., Chevalier, B., Mayoux, E., Charlemagne, D., Ollivier, L., Callens-el Amrani, F., Swynghedauw, B. British journal of clinical pharmacology. (1990) [Pubmed]
  6. Ontogeny of bile acid transport in brush border membrane vesicles from rat ileum. Moyer, M.S., Heubi, J.E., Goodrich, A.L., Balistreri, W.F., Suchy, F.J. Gastroenterology (1986) [Pubmed]
  7. Calcium induced release of mitochondrial cytochrome c by different mechanisms selective for brain versus liver. Andreyev, A., Fiskum, G. Cell Death Differ. (1999) [Pubmed]
  8. Ecto-ATPase of mammalian synaptosomes: identification and enzymic characterization. Nagy, A.K., Shuster, T.A., Delgado-Escueta, A.V. J. Neurochem. (1986) [Pubmed]
  9. Studies of rat brain metabolism using proton nuclear magnetic resonance: spectral assignments and monitoring of prolidase, acetylcholinesterase, and glutaminase. Middlehurst, C.R., King, G.F., Beilharz, G.R., Hunt, G.E., Johnson, G.F., Kuchel, P.W. J. Neurochem. (1984) [Pubmed]
  10. Angiotensin II receptor expression and inhibition in the chronically hypoxic rat lung. Zhao, L., al-Tubuly, R., Sebkhi, A., Owji, A.A., Nunez, D.J., Wilkins, M.R. Br. J. Pharmacol. (1996) [Pubmed]
  11. Agmatine (decarboxylated arginine) is synthesized and stored in astrocytes. Regunathan, S., Feinstein, D.L., Raasch, W., Reis, D.J. Neuroreport (1995) [Pubmed]
  12. BAY K 8644, a 1,4-dihydropyridine Ca2+ channel activator: dissociation of binding and functional effects in brain synaptosomes. Rampe, D., Janis, R.A., Triggle, D.J. J. Neurochem. (1984) [Pubmed]
  13. Ap4A and ADP-beta-S binding to P2 purinoceptors present on rat brain synaptic terminals. Pintor, J., Díaz-Rey, M.A., Miras-Portugal, M.T. Br. J. Pharmacol. (1993) [Pubmed]
  14. Ontogeny of beta-adrenergic receptors in the rat lung: effects of hypothyroidism. Whitsett, J.A., Darovec-Beckerman, C., Pollinger, J., Moore, J.J. Pediatr. Res. (1982) [Pubmed]
  15. Antagonistic actions of renal dopamine and 5-hydroxytryptamine: effects of amine precursors on the cell inward transfer and decarboxylation. Soares-da-Silva, P., Pinto-do-O, P.C. Br. J. Pharmacol. (1996) [Pubmed]
  16. N-methylation of nicotine enantiomers by human liver cytosol. Crooks, P.A., Godin, C.S. J. Pharm. Pharmacol. (1988) [Pubmed]
  17. Changes in [3H]-PK 11195 and [3H]-8-OH-DPAT binding following forebrain ischaemia in the gerbil. Kenny, B.A., MacKinnon, A.C., Spedding, M., Brown, C.M. Br. J. Pharmacol. (1993) [Pubmed]
  18. Inositol 1,4,5-triphosphate-stimulated calcium release from permeabilized cerebellar granule cells. Whitham, E.M., Challiss, R.A., Nahorski, S.R. Br. J. Pharmacol. (1991) [Pubmed]
  19. Alpha 2-adrenoceptor subtypes and imidazoline-like binding sites in the rat brain. Brown, C.M., MacKinnon, A.C., McGrath, J.C., Spedding, M., Kilpatrick, A.T. Br. J. Pharmacol. (1990) [Pubmed]
  20. [3H]-MK 912 binding delineates two alpha 2-adrenoceptor subtypes in rat CNS one of which is identical with the cloned pA2d alpha 2-adrenoceptor. Uhlén, S., Xia, Y., Chhajlani, V., Felder, C.C., Wikberg, J.E. Br. J. Pharmacol. (1992) [Pubmed]
  21. Inositol hexakisphosphate binding sites in rat heart and brain. Rowley, K.G., Gundlach, A.L., Cincotta, M., Louis, W.J. Br. J. Pharmacol. (1996) [Pubmed]
  22. Characterization and localization of atypical beta-adrenoceptors in rat ileum. Roberts, S.J., Russell, F.D., Molenaar, P., Summers, R.J. Br. J. Pharmacol. (1995) [Pubmed]
  23. Bay K 8644, modifier of calcium transport and energy metabolism in rat heart mitochondria: a new intracellular site of action. Baydoun, A.R., Markham, A., Morgan, R.M., Sweetman, A.J. Br. J. Pharmacol. (1990) [Pubmed]
  24. Impaired cyclic nucleotide-mediated vasorelaxation may contribute to closure of the human umbilical artery after birth. Renowden, S., Edwards, D.H., Griffith, T.M. Br. J. Pharmacol. (1992) [Pubmed]
  25. Constitutive activity of the delta-opioid receptor expressed in C6 glioma cells: identification of non-peptide delta-inverse agonists. Neilan, C.L., Akil, H., Woods, J.H., Traynor, J.R. Br. J. Pharmacol. (1999) [Pubmed]
  26. Characterization of the binding of DL-[3H]-2-amino-4-phosphonobutyrate to L-glutamate-sensitive sites on rat brain synaptic membranes. Butcher, S.P., Collins, J.F., Roberts, P.J. Br. J. Pharmacol. (1983) [Pubmed]
  27. Characterization of [125I]-endothelin-1 and [125I]-BQ3020 binding to rat cerebellar endothelin receptors. Widdowson, P.S., Kirk, C.N. Br. J. Pharmacol. (1996) [Pubmed]
  28. Chronic oral administration of Ocimum sanctum Linn. augments cardiac endogenous antioxidants and prevents isoproterenol-induced myocardial necrosis in rats. Sood, S., Narang, D., Dinda, A.K., Maulik, S.K. J. Pharm. Pharmacol. (2005) [Pubmed]
  29. Identification and distribution of 5-HT3 recognition sites in the rat gastrointestinal tract. Champaneria, S., Costall, B., Naylor, R.J., Robertson, D.W. Br. J. Pharmacol. (1992) [Pubmed]
  30. Antagonistic actions of renal dopamine and 5-hydroxytryptamine: increase in Na+, K(+)-ATPase activity in renal proximal tubules via activation of 5-HT1A receptors. Soares-da-Silva, P., Pinto-do-O, P.C., Bertorello, A.M. Br. J. Pharmacol. (1996) [Pubmed]
  31. Characterization of the binding of [3H]-CGS 19755: a novel N-methyl-D-aspartate antagonist with nanomolar affinity in rat brain. Murphy, D.E., Hutchison, A.J., Hurt, S.D., Williams, M., Sills, M.A. Br. J. Pharmacol. (1988) [Pubmed]
  32. Impaired renal function in obstructive jaundice: enhanced glomerular thromboxane synthesis and effects of thromboxane receptor blockade in bile duct-ligated rats. Kramer, H.J., Schwarting, K., Bäcker, A. Clin. Sci. (1995) [Pubmed]
  33. Regulation of VLDL secretion in primary culture of rat hepatocytes: involvement of cAMP and cAMP-dependent protein kinases. Björnsson, O.G., Sparks, J.D., Sparks, C.E., Gibbons, G.F. Eur. J. Clin. Invest. (1994) [Pubmed]
  34. Effects of dietary fluoride and magnesium supplements on cyclic adenosine monophosphate (cAMP), calcium and magnesium levels in aorta of genetically hypercholesterolaemic RICO rats. Luoma, H., Metsä-Ketelä, T., Jauhiainen, M., Alakuijala, P., Korhonen, A., Nevalainen, T. Scand. J. Clin. Lab. Invest. (1997) [Pubmed]
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