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Gene Review

Anxa5  -  annexin A5

Rattus norvegicus

Synonyms: Anchorin CII, Annexin A5, Annexin V, Annexin-5, Anx5, ...
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Disease relevance of Anxa5


High impact information on Anxa5

  • These alterations in annexin V suggest a role for domain 3 in calcium-triggered interaction with phospholipid membranes [6].
  • C5a blockade dramatically reduced thymocyte apoptosis as measured by thymic weight, binding of annexin V to thymocytes, and laddering of thymocyte DNA [7].
  • Blocking of Fas protein reduced the number of apoptotic cells in the liver; binding of annexin V to phosphatidylserine (PS) reduced the number of PMNs phagocytosed by Kupffer cells [8].
  • The presence of apoptosis was evaluated by morphological criteria, annexin V binding, and DNA fragmentation and quantified as the proportion of hypodiploid cells by flow cytometry [9].
  • Thrombin activation was also seen with apoptotic human VSMCs (AUC of 211 +/- 8 nmol x min/L; PT of 103 +/- 4 nmol/L) and was inhibited by annexin V (P < .0001 for AUC and PT) [10].

Chemical compound and disease context of Anxa5


Biological context of Anxa5

  • Here, we investigated in detail and correlated the adsorption kinetics of annexin A5 on SLBs, supported on silica and on mica, with the protein's 2D self-assembly behavior [15].
  • Herein, we screened an embryonic day 17 rat brain cDNA library by western blot using glutathione S-transferase-annexin V fusion protein as a probe and then isolated four clones showing binding to annexin V in a Ca2+ - and phospholipid-dependent manner [16].
  • Previous studies found that both human and chicken annexin V genes bear 13 exons and one promoter, which possess no TATA sequence or CCAAT box, and have a high GC content [17].
  • We also demonstrated the presence of at least four species of rat annexin V transcripts that were generated by distinct transcription initiation and alternative splicing [17].
  • We report here the isolation and characterization of the entire rat annexin V gene, which is shown to span about 40 kbp, and to consist of 14 exons, 13 introns and two promoters [17].

Anatomical context of Anxa5


Associations of Anxa5 with chemical compounds


Regulatory relationships of Anxa5


Other interactions of Anxa5

  • In contrast, camptothecin- and etoposide-induced killing is associated with robust increases in caspase 3 activation and annexin V staining [27].
  • Cell death, presumably by apoptosis as evidence by TUNEL and Annexin V binding, was observed following LPS/IFN-gamma, progenitors being more sensitive than differentiated cells [28].
  • Hyperosmolarity, however, sensitized hepatocytes toward CD95L-induced apoptosis, as assessed by annexin V staining and terminal deoxynucleotidyl transferase-mediated X-dUTP nick-end labeling (TUNEL) assay [29].
  • Thymocytes obtained 2 or 3 h after CLP and exposed in vitro to C5a, but not normal thymocytes, underwent increased apoptosis, as demonstrated by annexin-V binding, coinciding with increased activation of caspases 3, 6, and 8 [30].
  • Antibody was produced against the annexin consensus peptide, Lys-Ala-Met-Lys-Gly-Leu-Gly-Thr-Asp-Glu, which was derived from the sequence of several Ca2+/phospholipid-binding proteins including calpactin, lipocortin, endonexin II, 67-kDa calelectrin, lymphocyte 68-kDa protein, and protein II [31].

Analytical, diagnostic and therapeutic context of Anxa5


  1. An integrated physical and gene map of the 3.5-Mb chromosome 3p21.3 (AP20) region implicated in major human epithelial malignancies. Protopopov, A., Kashuba, V., Zabarovska, V.I., Muravenko, O.V., Lerman, M.I., Klein, G., Zabarovsky, E.R. Cancer Res. (2003) [Pubmed]
  2. Detection of cardiomyocyte death in a rat model of ischemia and reperfusion using 99mTc-labeled annexin V. Taki, J., Higuchi, T., Kawashima, A., Tait, J.F., Kinuya, S., Muramori, A., Matsunari, I., Nakajima, K., Tonami, N., Strauss, H.W. J. Nucl. Med. (2004) [Pubmed]
  3. Preparation and characterization of a disulfide-linked bioconjugate of annexin V with the B-chain of urokinase: an improved fibrinolytic agent targeted to phospholipid-containing thrombi. Tanaka, K., Einaga, K., Tsuchiyama, H., Tait, J.F., Fujikawa, K. Biochemistry (1996) [Pubmed]
  4. Hypoxia-induced apoptosis in cultured glomerular endothelial cells: involvement of mitochondrial pathways. Tanaka, T., Miyata, T., Inagi, R., Kurokawa, K., Adler, S., Fujita, T., Nangaku, M. Kidney Int. (2003) [Pubmed]
  5. Imaging cyclophosphamide-induced intramedullary apoptosis in rats using 99mTc-radiolabeled annexin V. Blankenberg, F.G., Naumovski, L., Tait, J.F., Post, A.M., Strauss, H.W. J. Nucl. Med. (2001) [Pubmed]
  6. Rat annexin V crystal structure: Ca(2+)-induced conformational changes. Concha, N.O., Head, J.F., Kaetzel, M.A., Dedman, J.R., Seaton, B.A. Science (1993) [Pubmed]
  7. Protective effects of anti-C5a in sepsis-induced thymocyte apoptosis. Guo, R.F., Huber-Lang, M., Wang, X., Sarma, V., Padgaonkar, V.A., Craig, R.A., Riedemann, N.C., McClintock, S.D., Hlaing, T., Shi, M.M., Ward, P.A. J. Clin. Invest. (2000) [Pubmed]
  8. Role of the liver in regulating numbers of circulating neutrophils. Shi, J., Gilbert, G.E., Kokubo, Y., Ohashi, T. Blood (2001) [Pubmed]
  9. 3-Hydroxy-3-methylglutaryl coenzyme a reductase and isoprenylation inhibitors induce apoptosis of vascular smooth muscle cells in culture. Guijarro, C., Blanco-Colio, L.M., Ortego, M., Alonso, C., Ortiz, A., Plaza, J.J., Díaz, C., Hernández, G., Egido, J. Circ. Res. (1998) [Pubmed]
  10. Thrombin generation by apoptotic vascular smooth muscle cells. Flynn, P.D., Byrne, C.D., Baglin, T.P., Weissberg, P.L., Bennett, M.R. Blood (1997) [Pubmed]
  11. Annexin V imaging of acute doxorubicin cardiotoxicity (apoptosis) in rats. Bennink, R.J., van den Hoff, M.J., van Hemert, F.J., de Bruin, K.M., Spijkerboer, A.L., Vanderheyden, J.L., Steinmetz, N., van Eck-Smit, B.L. J. Nucl. Med. (2004) [Pubmed]
  12. Adenosine A(3) receptor activation protects the myocardium from reperfusion/reoxygenation injury. Maddock, H.L., Mocanu, M.M., Yellon, D.M. Am. J. Physiol. Heart Circ. Physiol. (2002) [Pubmed]
  13. Localization of annexin V in rat normal kidney and experimental glomerulonephritis. Matsuda, R., Kaneko, N., Horikawa, Y., Chiwaki, F., Shinozaki, M., Ieiri, T., Suzuki, T., Ogawa, N. Research in experimental medicine. Zeitschrift für die gesamte experimentelle Medizin einschliesslich experimenteller Chirurgie. (2001) [Pubmed]
  14. Inhibitory effects of 1-methyl-4-phenylpyridinium on glutamate uptake into cultured C6 glioma cells. Yao, H.H., Ding, J.H., He, H.R., Hu, G. Acta Pharmacol. Sin. (2004) [Pubmed]
  15. On the kinetics of adsorption and two-dimensional self-assembly of annexin A5 on supported lipid bilayers. Richter, R.P., Him, J.L., Tessier, B., Tessier, C., Brisson, A.R. Biophys. J. (2005) [Pubmed]
  16. Molecular cloning and characterization of annexin V-binding proteins with highly hydrophilic peptide structure. Ohsawa, K., Imai, Y., Ito, D., Kohsaka, S. J. Neurochem. (1996) [Pubmed]
  17. Structure of rat annexin V gene and molecular diversity of its transcripts. Imai, Y., Kohsaka, S. Eur. J. Biochem. (1995) [Pubmed]
  18. Annexin expressions are temporally and spatially regulated during rat hepatocyte differentiation. Della Gaspera, B., Braut-Boucher, F., Bomsel, M., Chatelet, F., Guguen-Guillouzo, C., Font, J., Weinman, J., Weinman, S. Dev. Dyn. (2001) [Pubmed]
  19. Differential expression of annexins I-VI in the rat dorsal root ganglia and spinal cord. Naciff, J.M., Kaetzel, M.A., Behbehani, M.M., Dedman, J.R. J. Comp. Neurol. (1996) [Pubmed]
  20. Association of annexin V with prolactin in the rat anterior pituitary gland. Kawaminami, M., Tanaka, K., Asawa, T., Osugi, S., Kawauchi, H., Kurusu, S., Hashimoto, I. Biochem. Biophys. Res. Commun. (1992) [Pubmed]
  21. Thrombopoietin protects against in vitro and in vivo cardiotoxicity induced by doxorubicin. Li, K., Sung, R.Y., Huang, W.Z., Yang, M., Pong, N.H., Lee, S.M., Chan, W.Y., Zhao, H., To, M.Y., Fok, T.F., Li, C.K., Wong, Y.O., Ng, P.C. Circulation (2006) [Pubmed]
  22. Interfacial basic cluster in annexin V couples phospholipid binding and trimer formation on membrane surfaces. Mo, Y., Campos, B., Mealy, T.R., Commodore, L., Head, J.F., Dedman, J.R., Seaton, B.A. J. Biol. Chem. (2003) [Pubmed]
  23. Mutational and crystallographic analyses of interfacial residues in annexin V suggest direct interactions with phospholipid membrane components. Campos, B., Mo, Y.D., Mealy, T.R., Li, C.W., Swairjo, M.A., Balch, C., Head, J.F., Retzinger, G., Dedman, J.R., Seaton, B.A. Biochemistry (1998) [Pubmed]
  24. Lipocortin V may function as a signaling protein for vascular endothelial growth factor receptor-2/Flk-1. Wen, Y., Edelman, J.L., Kang, T., Sachs, G. Biochem. Biophys. Res. Commun. (1999) [Pubmed]
  25. Binding and phagocytosis of apoptotic vascular smooth muscle cells is mediated in part by exposure of phosphatidylserine. Bennett, M.R., Gibson, D.F., Schwartz, S.M., Tait, J.F. Circ. Res. (1995) [Pubmed]
  26. Effects of interleukin-15 on suppression of rat pancreatic islets in vitro induced by proinflammatory cytokines. Wallström, J., Andersson, A.K., Sandler, S. Immunol. Lett. (2003) [Pubmed]
  27. Pro- and Antiapoptotic Proteins Regulate Apoptosis but Do Not Protect Against Cytokine-Mediated Cytotoxicity in Rat Islets and {beta}-Cell Lines. Collier, J.J., Fueger, P.T., Hohmeier, H.E., Newgard, C.B. Diabetes (2006) [Pubmed]
  28. LPS/IFN-gamma cytotoxicity in oligodendroglial cells: role of nitric oxide and protection by the anti-inflammatory cytokine IL-10. Molina-Holgado, E., Vela, J.M., Arévalo-Martín, A., Guaza, C. Eur. J. Neurosci. (2001) [Pubmed]
  29. Hyperosmolarity triggers CD95 membrane trafficking and sensitizes rat hepatocytes toward CD95L-induced apoptosis. Reinehr, R., Graf, D., Fischer, R., Schliess, F., Häussinger, D. Hepatology (2002) [Pubmed]
  30. C5a receptor and thymocyte apoptosis in sepsis. Riedemann, N.C., Guo, R.F., Laudes, I.J., Keller, K., Sarma, V.J., Padgaonkar, V., Zetoune, F.S., Ward, P.A. FASEB J. (2002) [Pubmed]
  31. Differential tissue expression of three 35-kDa annexin calcium-dependent phospholipid-binding proteins. Kaetzel, M.A., Hazarika, P., Dedman, J.R. J. Biol. Chem. (1989) [Pubmed]
  32. Normal development of the outflow tract in the rat. Ya, J., van den Hoff, M.J., de Boer, P.A., Tesink-Taekema, S., Franco, D., Moorman, A.F., Lamers, W.H. Circ. Res. (1998) [Pubmed]
  33. Fc gammaRII (CD32) is linked to apoptotic pathways in murine granulocyte precursors and mature eosinophils. de Andrés, B., Mueller, A.L., Blum, A., Weinstock, J., Verbeek, S., Sandor, M., Lynch, R.G. Blood (1997) [Pubmed]
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