The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

Mme  -  membrane metallo-endopeptidase

Rattus norvegicus

Synonyms: Atriopeptidase, CD10, Enkephalinase, NEP, Nep, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of Mme


Psychiatry related information on Mme


High impact information on Mme


Chemical compound and disease context of Mme


Biological context of Mme


Anatomical context of Mme

  • Plasma NEP activity, tissue NEP activity and its protein and mRNA expression in the left ventricle, aorta, jejunum and lung in the late phase of shock (LS) rats were lower than those in ES and the control, but no statistical change of NEP activity in the kidney was observed [2].
  • Immunolabeling density of NEP in the airway epithelium largely disappeared 1 h after insult due to acute cell damage and shedding [3].
  • Because enkephalinase has it active site located extracellularly and is thus an ectopeptidase, we suggest that the N-terminal transmembrane region of the enzyme anchors the protein in membranes and that the majority of the protein, including the carboxy terminus, is extracellular [18].
  • ASA decreased NEP activity in a dose-dependent manner, and decreased Western-detectable NEP protein in plasma membranes [22].
  • We assessed NEP activity in an osteoblastic cell line, PyMS, by cleavage of N-Dansyl-d-Ala-Gly-p-nitro-Phe-Gly to Dansyl-d-Ala-Gly [22].

Associations of Mme with chemical compounds


Physical interactions of Mme


Enzymatic interactions of Mme


Regulatory relationships of Mme


Other interactions of Mme


Analytical, diagnostic and therapeutic context of Mme

  • The relative abundance of NEP mRNA was determined by RT-PCR in the right atrium (RA), right ventricle (RV), left atrium (LA), left ventricle (LV) and interventricular septum (IVS) in 2-month-old (young) and 12-month-old (advanced-age adult) Wistar Kyoto (WKY) rats [19].
  • The cellular localization of rat brain enkephalinase was studied after induction of selective unilateral lesions using in vitro quantitative autoradiography of the specific binding of the enzyme inhibitor [3H]-N-[(2RS)-3-hydroxyaminocarbonyl-2-benzyl-1-oxopropyl]glycine ([3H]HACBO-Gly) [31].
  • The association of enkephalinase with delta and mu opioid receptors in these areas is consistent with the observed role of the enzyme in regulating the effects of opioid peptides in striatal dopamine release and analgesia, respectively [32].
  • Therefore, we examined the effect of an NEP inhibitor, phosphoramidon, on myocardial perfusion in rats after (1) stimulating sensory nerves with capsaicin and (2) inducing myocardial hypoperfusion with isoproterenol, with or without pretreatment with selective antagonists of the substance P (NK1) and bradykinin (B2) receptors [33].
  • The fine structural distribution of the enzyme-neutral endopeptidase EC (enkephalinase) was examined by immunoradioautography (using an iodinated monoclonal antibody) and peroxidase immunocytochemistry (using the same probe in nonradioactive form) in the neostriatum of the rat [34].


  1. Relationship between the contents of adrenomedullin and distributions of neutral endopeptidase in blood and tissues of spontaneously hypertensive rats. Jiang, W., Jiang, H.F., Pan, C.S., Cai, D.Y., Qi, Y.F., Pang, Y.Z., Tang, C.S. Hypertens. Res. (2004) [Pubmed]
  2. Relationship between contents of adrenomedullin and distributions of neutral endopeptidase in blood and tissues of rats in septic shock. Jiang, W., Jiang, H.F., Cai, D.Y., Pan, C.S., Qi, Y.F., Pang, Y.Z., Tang, C.S. Regul. Pept. (2004) [Pubmed]
  3. Substance P and neutral endopeptidase in development of acute respiratory distress syndrome following fire smoke inhalation. Wong, S.S., Sun, N.N., Lantz, R.C., Witten, M.L. Am. J. Physiol. Lung Cell Mol. Physiol. (2004) [Pubmed]
  4. Linkage and microarray analyses of susceptibility genes in ACI/Seg rats: a model for prostate cancers in the aged. Yamashita, S., Suzuki, S., Nomoto, T., Kondo, Y., Wakazono, K., Tsujino, Y., Sugimura, T., Shirai, T., Homma, Y., Ushijima, T. Cancer Res. (2005) [Pubmed]
  5. Effect of mixed (RB 38A) and selective (RB 38B) inhibitors of enkephalin degrading enzymes on a model of depression in the rat. Tejedor-Real, P., Micó, J.A., Maldonado, R., Roques, B.P., Gibert-Rahola, J. Biol. Psychiatry (1993) [Pubmed]
  6. Novel selective inhibitors of neutral endopeptidase for the treatment of female sexual arousal disorder. Synthesis and activity of functionalized glutaramides. Pryde, D.C., Maw, G.N., Planken, S., Platts, M.Y., Sanderson, V., Corless, M., Stobie, A., Barber, C.G., Russell, R., Foster, L., Barker, L., Wayman, C., Van Der Graaf, P., Stacey, P., Morren, D., Kohl, C., Beaumont, K., Coggon, S., Tute, M. J. Med. Chem. (2006) [Pubmed]
  7. Amelioration of naloxone-precipitated opioid withdrawal symptoms by peripheral administration of the enkephalinase inhibitor acetorphan. Livingston, S.J., Sewell, R.D., Rooney, K.F., Smith, H.J. Psychopharmacology (Berl.) (1988) [Pubmed]
  8. Effects of SCH 32615, an enkephalinase inhibitor, on D-1 and D-2 dopamine receptor-mediated behaviors. Marin, C., Chase, T.N. Neuropharmacology (1995) [Pubmed]
  9. Importance of delta opioid receptors in maintaining high alcohol drinking. Froehlich, J.C., Zweifel, M., Harts, J., Lumeng, L., Li, T.K. Psychopharmacology (Berl.) (1991) [Pubmed]
  10. Selective protection of methionine enkephalin released from brain slices by enkephalinase inhibition. Patey, G., De La Baume, S., Schwartz, J.C., Gros, C., Roques, B., Fournie-Zaluski, M.C., Soroca-Lucas, E. Science (1981) [Pubmed]
  11. Neutral endopeptidase and kininase II mediate glucocorticoid inhibition of neurogenic inflammation in the rat trachea. Piedimonte, G., McDonald, D.M., Nadel, J.A. J. Clin. Invest. (1991) [Pubmed]
  12. Dual ACE and NEP inhibitor MDL-100,240 prevents and regresses severe angiotensin II-dependent hypertension partially through bradykinin type 2 receptor. Rossi, G.P., Cavallin, M., Rizzoni, D., Bova, S., Mazzocchi, G., Agabiti-Rosei, E., Nussdorfer, G.G., Pessina, A.C. J. Hypertens. (2002) [Pubmed]
  13. The vasopeptidase inhibitor AVE7688 ameliorates Type 2 diabetic nephropathy. Schäfer, S., Linz, W., Vollert, H., Biemer-Daub, G., Rütten, H., Bleich, M., Busch, A.E. Diabetologia (2004) [Pubmed]
  14. Pulmonary and renal neutral endopeptidase EC in rats with experimental heart failure. Abassi, Z.A., Kotob, S., Golomb, E., Pieruzzi, F., Keiser, H.R. Hypertension (1995) [Pubmed]
  15. Dual inhibition of neutral endopeptidase and angiotensin-converting enzyme in rats with hypertension and diabetes mellitus. Tikkanen, T., Tikkanen, I., Rockell, M.D., Allen, T.J., Johnston, C.I., Cooper, M.E., Burrell, L.M. Hypertension (1998) [Pubmed]
  16. Dual angiotensin-converting enzyme/neutral endopeptidase inhibition on cardiac and renal fibrosis and inflammation in DOCA-salt hypertensive rats. Pu, Q., Amiri, F., Gannon, P., Schiffrin, E.L. J. Hypertens. (2005) [Pubmed]
  17. Identification of the active-site arginine in rat neutral endopeptidase 24.11 (enkephalinase) as arginine 102 and analysis of a glutamine 102 mutant. Bateman, R.C., Jackson, D., Slaughter, C.A., Unnithan, S., Chai, Y.G., Moomaw, C., Hersh, L.B. J. Biol. Chem. (1989) [Pubmed]
  18. Molecular cloning and amino acid sequence of rat enkephalinase. Malfroy, B., Schofield, P.R., Kuang, W.J., Seeburg, P.H., Mason, A.J., Henzel, W.J. Biochem. Biophys. Res. Commun. (1987) [Pubmed]
  19. Intracardiac expression of neutral endopeptidase. Raizada, V., Luo, W., Skipper, B.J., McGuire, P.G. Mol. Cell. Biochem. (2002) [Pubmed]
  20. The first preclinical pharmacotoxicological safety assessment of CGS 35601, a triple vasopeptidase inhibitor, in chronically instrumented, conscious, and unrestrained spontaneously hypertensive rats. Daull, P., Lepage, R., Benrezzak, O., Cayer, J., Beaudoin, M., Belleville, K., Blouin, A., Sirois, P., Nantel, F., Jeng, A.Y., Battistini, B. Drug and chemical toxicology. (2006) [Pubmed]
  21. Triple VPI CGS 35601 reduces high blood pressure in low-renin, high-salt Dahl salt-sensitive rats. Daull, P., Blouin, A., Belleville, K., Beaudoin, M., Arsenault, D., Leonard, H., Sirois, P., Nantel, F., Jeng, A.Y., Battistini, B. Exp. Biol. Med. (Maywood) (2006) [Pubmed]
  22. Ascorbic acid decreases neutral endopeptidase activity in cultured osteoblastic cells. Schmid, C., Ghirlanda-Keller, C., Gosteli-Peter, M. Regul. Pept. (2005) [Pubmed]
  23. Omapatrilat: a unique new agent for the treatment of cardiovascular disease. Nawarskas, J.J., Anderson, J.R. Heart disease (Hagerstown, Md.) (2000) [Pubmed]
  24. Hypertonic saline increases vascular permeability in the rat trachea by producing neurogenic inflammation. Umeno, E., McDonald, D.M., Nadel, J.A. J. Clin. Invest. (1990) [Pubmed]
  25. Rat endothelin-converting enzyme-1 forms a dimer through Cys412 with a similar catalytic mechanism and a distinct substrate binding mechanism compared with neutral endopeptidase-24.11. Shimada, K., Takahashi, M., Turner, A.J., Tanzawa, K. Biochem. J. (1996) [Pubmed]
  26. Fluorescent histochemical localization of neutral endopeptidase-24.11 (enkephalinase) in the rat spinal cord. Back, S.A., Gorenstein, C. J. Comp. Neurol. (1989) [Pubmed]
  27. Assessment of the role of "enkephalinase" in cholecystokinin inactivation. Zuzel, K.A., Rose, C., Schwartz, J.C. Neuroscience (1985) [Pubmed]
  28. The natriuretic mechanisms of neutral endopeptidase inhibitor in rats. Shimamoto, K., Ura, N., Iimura, O. Braz. J. Med. Biol. Res. (1994) [Pubmed]
  29. Androgen-induced growth inhibition of androgen receptor expressing androgen-independent prostate cancer cells is mediated by increased levels of neutral endopeptidase. Shen, R., Sumitomo, M., Dai, J., Harris, A., Kaminetzky, D., Gao, M., Burnstein, K.L., Nanus, D.M. Endocrinology (2000) [Pubmed]
  30. Tachykinin receptor and neutral endopeptidase gene expression in the rat uterus: characterization and regulation in response to ovarian steroid treatment. Pinto, F.M., Armesto, C.P., Magraner, J., Trujillo, M., Martín, J.D., Candenas, M.L. Endocrinology (1999) [Pubmed]
  31. Neuronal localization of the neutral endopeptidase 'enkephalinase' in rat brain revealed by lesions and autoradiography. Waksman, G., Hamel, E., Delay-Goyet, P., Roques, B.P. EMBO J. (1986) [Pubmed]
  32. Autoradiographic comparison of the distribution of the neutral endopeptidase "enkephalinase" and of mu and delta opioid receptors in rat brain. Waksman, G., Hamel, E., Fournié-Zaluski, M.C., Roques, B.P. Proc. Natl. Acad. Sci. U.S.A. (1986) [Pubmed]
  33. Neutral endopeptidase in the heart. Neutral endopeptidase inhibition prevents isoproterenol-induced myocardial hypoperfusion in rats by reducing bradykinin degradation. Piedimonte, G., Nadel, J.A., Long, C.S., Hoffman, J.I. Circ. Res. (1994) [Pubmed]
  34. Electron microscopic localization of immunoreactive enkephalinase (EC in the neostriatum of the rat. Marcel, D., Pollard, H., Verroust, P., Schwartz, J.C., Beaudet, A. J. Neurosci. (1990) [Pubmed]
WikiGenes - Universities