The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

NES  -  nestin

Homo sapiens

Synonyms: FLJ21841, Nbla00170, Nestin
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of NES


Psychiatry related information on NES


High impact information on NES

  • Interestingly, an NES mutant of c-Fos, but not wild-type, substitutes ERK5 activity for HeLa cell proliferation [11].
  • The number of immature glial cell fibers, expressing nestin, was not affected, but the proportion of mature glial cell fibers, expressing glial fibrillary acidic protein, was significantly decreased by both LID and MMI treatment of the dams [12].
  • A nestin scaffold links Cdk5/p35 signaling to oxidant-induced cell death [13].
  • The intermediate filament protein, nestin, has been implicated as an organizer of survival-determining signaling molecules [13].
  • When nestin expression was related to the sensitivity of neural progenitor cells to oxidant-induced apoptosis, nestin displayed a distinct cytoprotective effect [13].

Chemical compound and disease context of NES

  • Full-length atrophin-1 with 65 polyglutamine repeats and mutations inactivating the NES also yielded increased nuclear localization and increased toxicity [14].
  • A construct with 65 glutamine repeats encoding an N-terminal fragment (which removes an NES) of atrophin-1 similar in size to the truncation product in DRPLA patient tissue, showed increased nuclear labeling, and an increase in cellular toxicity, compared with a similar fragment with 26 glutamines [14].
  • Outside any period of acute exposure, the workers answered a self-administered questionnaire on neurotoxic symptoms, EUROQUEST, and performed six psychometric tests on a computer-assisted version of battery NES [15].
  • Apart from that, a significant change was observed in 5/41 parameters being monitored (the pair t-test): I) decrease in the total NES score, II) decrease in the sensorial integration subscale NES score, III) increase in psychomotor speed, IV) decrease in auditory reaction time, V) increase in basal cortisol [16].
  • The complex retroviruses encode a Rev protein that uses a leucine-rich NES to interact with CRM1 and the U snRNA-export pathway [17].

Biological context of NES


Anatomical context of NES

  • Subcellular fractionation and immunoblot studies indicate that nestin is present in the nucleus as well as in the cytoplasm of neuroblastoma cell lines [1].
  • We observed that 5-bromodeoxyuridine (BrdU) + nestin double-labeled neural precursor cells increased in the spinal cords of Tg mice compared with non-Tg mice, with a much greater increase produced by EGF and FGF2 treatment [19].
  • HLSCs were also positive for vimentin and nestin, a stem cell marker [20].
  • In conclusion, nestin is expressed in the human kidney from the first steps of glomerulogenesis within podocytes, mesangial, and endothelial cells [21].
  • Nestin was also detected in developing glomeruli within immature podocytes and a few other cells [21].

Associations of NES with chemical compounds

  • METHODS: Formalin fixed, paraffin processed sections from two human foetuses, 16 brain tumours of both neuronal and glial lineage and two metastatic tumours were immunostained with polyclonal antibody to nestin [22].
  • To further explore the neuroprotective mechanisms underlying HB1.F3 cell transplantation, we performed cell culture studies and found that a modest number of HB1.F3 cells were TH and dopamine and cAMP-regulated phosphoprotein 32 positive, although most cells were nestin positive, suggesting a mixed population of mature and immature cells [23].
  • The induced cells were observed for morphological properties and detected for expressions of type II collagen, triglyceride or neuron-specific enolase and nestin [24].
  • A 150-amino-acid fragment derived from the cloned nestin cDNA was coupled to glutathione S-transferase and used as an immunogen to generate a rabbit polyclonal antiserum that selectively detects human nestin [25].
  • CONCLUSIONS: A subpopulation of human corneal stem cells exhibit neuronal properties in vitro, as evidenced by immunoreactivity to nestin, GABA receptor, glycine receptor, and serotonin receptor, as well as functional neurophysiological responses to GABA and kainic acid [26].

Physical interactions of NES


Co-localisations of NES


Regulatory relationships of NES


Other interactions of NES


Analytical, diagnostic and therapeutic context of NES


  1. Nestin is a potential mediator of malignancy in human neuroblastoma cells. Thomas, S.K., Messam, C.A., Spengler, B.A., Biedler, J.L., Ross, R.A. J. Biol. Chem. (2004) [Pubmed]
  2. Nestin expression in reactive astrocytes of human pathology. Tamagno, I., Schiffer, D. J. Neurooncol. (2006) [Pubmed]
  3. Constitutive phosphorylation of Janus kinase 2 in the GL15 glioblastoma derived human cell line. Sciaccaluga, M., Gianfranceschi, G.L., Rocco, S., Germano, G., Roti, G., Gorello, P., La Starza, R., Castigli, E. Oncol. Rep. (2007) [Pubmed]
  4. Redefining the concept of reactive astrocytes as cells that remain within their unique domains upon reaction to injury. Wilhelmsson, U., Bushong, E.A., Price, D.L., Smarr, B.L., Phung, V., Terada, M., Ellisman, M.H., Pekny, M. Proc. Natl. Acad. Sci. U.S.A. (2006) [Pubmed]
  5. Roles for the stem cell associated intermediate filament Nestin in prostate cancer migration and metastasis. Kleeberger, W., Bova, G.S., Nielsen, M.E., Herawi, M., Chuang, A.Y., Epstein, J.I., Berman, D.M. Cancer Res. (2007) [Pubmed]
  6. Beta-cell differentiation during human development does not rely on nestin-positive precursors: implications for stem cell-derived replacement therapy. Piper, K., Ball, S.G., Turnpenny, L.W., Brickwood, S., Wilson, D.I., Hanley, N.A. Diabetologia (2002) [Pubmed]
  7. Expression of nestin in Purkinje cells in patients with Creutzfeldt-Jakob disease. Mizuno, Y., Takeuchi, T., Takatama, M., Okamoto, K. Neurosci. Lett. (2003) [Pubmed]
  8. Computerized assessment of human neurotoxicity: sensitivity to nitrous oxide exposure. Greenberg, B.D., Moore, P.A., Letz, R., Baker, E.L. Clin. Pharmacol. Ther. (1985) [Pubmed]
  9. Neurobehavioral Evaluation System (NES): comparative performance of 2nd-, 4th-, and 8th-grade Czech children. Otto, D.A., Skalik, I., House, D.E., Hudnell, H.K. Neurotoxicology and teratology. (1996) [Pubmed]
  10. A comparison of NES2 and traditional neuropsychological tests in a neurologic patient sample. Krengel, M., White, R.F., Diamond, R., Letz, R., Cyrus, P., Durso, R. Neurotoxicology and teratology. (1996) [Pubmed]
  11. Spatiotemporal Regulation of c-Fos by ERK5 and the E3 Ubiquitin Ligase UBR1, and Its Biological Role. Sasaki, T., Kojima, H., Kishimoto, R., Ikeda, A., Kunimoto, H., Nakajima, K. Mol. Cell (2006) [Pubmed]
  12. Early effects of iodine deficiency on radial glial cells of the hippocampus of the rat fetus. A model of neurological cretinism. Martínez-Galán, J.R., Pedraza, P., Santacana, M., Escobar del Ray, F., Morreale de Escobar, G., Ruiz-Marcos, A. J. Clin. Invest. (1997) [Pubmed]
  13. A nestin scaffold links Cdk5/p35 signaling to oxidant-induced cell death. Sahlgren, C.M., Pallari, H.M., He, T., Chou, Y.H., Goldman, R.D., Eriksson, J.E. EMBO J. (2006) [Pubmed]
  14. Nuclear localization of a non-caspase truncation product of atrophin-1, with an expanded polyglutamine repeat, increases cellular toxicity. Nucifora, F.C., Ellerby, L.M., Wellington, C.L., Wood, J.D., Herring, W.J., Sawa, A., Hayden, M.R., Dawson, V.L., Dawson, T.M., Ross, C.A. J. Biol. Chem. (2003) [Pubmed]
  15. Neurobehavioral disturbances arising from occupational toluene exposure. Chouanière, D., Wild, P., Fontana, J.M., Héry, M., Fournier, M., Baudin, V., Subra, I., Rousselle, D., Toamain, J.P., Saurin, S., Ardiot, M.R. Am. J. Ind. Med. (2002) [Pubmed]
  16. The changes of biological markers and treatment efficacy in schizophrenia. Cesková, E., Drybcák, P., Hrobar, P., Lorenc, M., Hana, n.u.l.l., Procházková, n.u.l.l., Spacek, J. Prog. Neuropsychopharmacol. Biol. Psychiatry (2001) [Pubmed]
  17. RNA export: insights from viral models. Harris, M.E., Hope, T.J. Essays Biochem. (2000) [Pubmed]
  18. Human amniotic fluid-derived stem cells have characteristics of multipotent stem cells. Kim, J., Lee, Y., Kim, H., Hwang, K.J., Kwon, H.C., Kim, S.K., Cho, D.J., Kang, S.G., You, J. Cell Prolif. (2007) [Pubmed]
  19. Intrathecal injection of epidermal growth factor and fibroblast growth factor 2 promotes proliferation of neural precursor cells in the spinal cords of mice with mutant human SOD1 gene. Ohta, Y., Nagai, M., Nagata, T., Murakami, T., Nagano, I., Narai, H., Kurata, T., Shiote, M., Shoji, M., Abe, K. J. Neurosci. Res. (2006) [Pubmed]
  20. Isolation and characterization of a stem cell population from adult human liver. Herrera, M.B., Bruno, S., Buttiglieri, S., Tetta, C., Gatti, S., Deregibus, M.C., Bussolati, B., Camussi, G. Stem Cells (2006) [Pubmed]
  21. Nestin expression in adult and developing human kidney. Bertelli, E., Regoli, M., Fonzi, L., Occhini, R., Mannucci, S., Ermini, L., Toti, P. J. Histochem. Cytochem. (2007) [Pubmed]
  22. Expression of nestin - a stem cell associated intermediate filament in human CNS tumours. Rani, S.B., Mahadevan, A., Anilkumar, S.R., Raju, T.R., Shankar, S.K. Indian J. Med. Res. (2006) [Pubmed]
  23. Transplantation of human neural stem cells exerts neuroprotection in a rat model of Parkinson's disease. Yasuhara, T., Matsukawa, N., Hara, K., Yu, G., Xu, L., Maki, M., Kim, S.U., Borlongan, C.V. J. Neurosci. (2006) [Pubmed]
  24. Ex vivo expansion and pluripotential differentiation of cryopreserved human bone marrow mesenchymal stem cells. Xiang, Y., Zheng, Q., Jia, B.B., Huang, G.P., Xu, Y.L., Wang, J.F., Pan, Z.J. Journal of Zhejiang University. Science. B. (2007) [Pubmed]
  25. Coexpression of nestin in neural and glial cells in the developing human CNS defined by a human-specific anti-nestin antibody. Messam, C.A., Hou, J., Major, E.O. Exp. Neurol. (2000) [Pubmed]
  26. Human corneal stem cells display functional neuronal properties. Seigel, G.M., Sun, W., Salvi, R., Campbell, L.M., Sullivan, S., Reidy, J.J. Mol. Vis. (2003) [Pubmed]
  27. Müller glial cells express nestin coupled with glial fibrillary acidic protein in experimentally induced glaucoma in the rat retina. Xue, L.P., Lu, J., Cao, Q., Hu, S., Ding, P., Ling, E.A. Neuroscience (2006) [Pubmed]
  28. Asymmetric localization of LGN but not AGS3, two homologs of Drosophila pins, in dividing human neural progenitor cells. Fuja, T.J., Schwartz, P.H., Darcy, D., Bryant, P.J. J. Neurosci. Res. (2004) [Pubmed]
  29. A population of human brain parenchymal cells express markers of glial, neuronal and early neural cells and differentiate into cells of neuronal and glial lineages. Rieske, P., Azizi, S.A., Augelli, B., Gaughan, J., Krynska, B. Eur. J. Neurosci. (2007) [Pubmed]
  30. Adult bone marrow stromal cells differentiate into neural cells in vitro. Sanchez-Ramos, J., Song, S., Cardozo-Pelaez, F., Hazzi, C., Stedeford, T., Willing, A., Freeman, T.B., Saporta, S., Janssen, W., Patel, N., Cooper, D.R., Sanberg, P.R. Exp. Neurol. (2000) [Pubmed]
  31. Intermediate filament nestin expressions in human cord blood monocytes (HCMNCs). Ha, Y., Lee, J.E., Kim, K.N., Cho, Y.E., Yoon, D.H. Acta neurochirurgica. (2003) [Pubmed]
  32. Molecular aspects of tooth pathogenesis and repair: in vivo and in vitro models. About, I., Mitsiadis, T.A. Adv. Dent. Res. (2001) [Pubmed]
  33. Identification of an NTF2-related factor that binds Ran-GTP and regulates nuclear protein export. Black, B.E., Lévesque, L., Holaska, J.M., Wood, T.C., Paschal, B.M. Mol. Cell. Biol. (1999) [Pubmed]
  34. Neurogenic neuroepithelial and radial glial cells generated from six human embryonic stem cell lines in serum-free suspension and adherent cultures. Nat, R., Nilbratt, M., Narkilahti, S., Winblad, B., Hovatta, O., Nordberg, A. Glia (2007) [Pubmed]
  35. Characterization and isolation of promoter-defined nestin-positive cells from the human fetal pancreas. Humphrey, R.K., Bucay, N., Beattie, G.M., Lopez, A., Messam, C.A., Cirulli, V., Hayek, A. Diabetes (2003) [Pubmed]
  36. Human astrocytes can be induced to differentiate into cells with neuronal phenotype. Pillai, R., Scintu, F., Scorciapino, L., Carta, M., Murru, L., Biggio, G., Cabras, S., Reali, C., Sogos, V. Exp. Cell Res. (2006) [Pubmed]
  37. An evolutionarily conserved region in the second intron of the human nestin gene directs gene expression to CNS progenitor cells and to early neural crest cells. Lothian, C., Lendahl, U. Eur. J. Neurosci. (1997) [Pubmed]
WikiGenes - Universities