Disease relevance of LIMA1

• The expression of EPLIN-alpha is either down-regulated or lost in the majority of oral cancer cell lines (8/8), prostate cancer cell lines (4/4) and xenograft tumors (3/3), and breast cancer cell lines (5/6) [1].
• In the majority of human breast and prostate cancer cell lines, the expression of EPLIN-alpha is significantly reduced, while the expression of EPLIN-beta is either up-regulated or unchanged [2].
• Loss of epidermal cohesion in normal skin explants incubated with pemphigus foliaceous IgG was dependent upon the addition of plasminogen and was inhibited by aprotinin or lima bean trypsin inhibitor, which indicated that plasmin is the active enzyme in producing acantholysis [3].
• Vibrio cholerae non-O1 isolated from five people with diarrhoea in Lima [4].
• We studied men with (study group) and without excessive erythrocytosis (packed-cell volume >65%) living in Cerro de Pasco, Peru (altitude 4300 m), and compared them with controls living in Lima, Peru (at sea-level) [5].

Psychiatry related information on LIMA1

• CONCLUSIONS: HIV infection risk among pregnant women in Lima depends largely on their male partners' risk behaviors [6].
• A 36-year-old woman with no significant medical or familial history flew economy class from Lima, Peru, to Madrid, Spain. On disembarkation she presented sudden dyspnea and a depressed level of consciousness, global aphasia, and right hemiparesis [7].
• The participants were 532 students in the sixth, seventh, and eighth grades from Shawnee Middle School in Lima, Ohio. The students were asked to a complete a questionnaire (CANKAP--Comprehensive Assessment of Nutrition Knowledge, Attitudes, and Practices), which measured nutrition knowledge and eating behavior, and is reproduced here [8].
• The classical leucotomy of Moniz and Lima, with some modifications, was used for treatment of schizophrenia and severe pain [9].
• Socioeconomic conditions, food habits and formulated food programs in the Pueblos Jóvenes of Lima, Peru [10].

High impact information on LIMA1

• An A alpha-arginine-141 to serine substitution has been identified in a homozygous dysfibrinogen, fibrinogen Lima, associated with impaired fibrin polymerization [11].
• INTRODUCTION: To investigate whether the El Niño phenomenon and ambient temperature had an effect on the epidemiology of childhood diarrhoea, we analysed data on daily number of admissions of children with diarrhoea to the Oral Rehydration Unit of the Instituto de Salud del Niño in Lima, Peru, between January, 1993, and November, 1998 [12].
• El Niño and diarrhoea and dehydration in Lima, Peru [13].
• We obtained serum and/or CSF samples from 204 consecutive patients admitted to a neurological ward in Lima, Peru, and looked for antibodies specific for T solium with the enzyme-linked immunoelectrotransfer blot (EITB) assay [14].
• The findings show that the prevalence of H pylori infection is high among young Peruvian children and that the municipal water supply seems to be an important source of infection among Lima children from families of both low and high socioeconomic status [15].

Chemical compound and disease context of LIMA1

• The authors measured maternal third trimester plasma folate, vitamin B(12), and homocyst(e)ine concentrations among 125 women with preeclampsia and 179 normotensive women in Lima, Peru (1997-1998), to determine whether these analytes were associated with the occurrence of preeclampsia [16].
• We conducted a randomized double-blinded study in Lima, Peru, to assess the tolerability and efficacy of a single 250-mg dose of ciprofloxacin in preventing diarrhea and Vibrio cholerae O1 infection among household contacts of bacteriologically confirmed index cases [17].
• This case-control study was conducted in Lima, Peru, from June 1997 through January 1998 to assess whether plasma concentrations of carotenoids (alpha-carotene, beta-carotene, lycopene, lutein, zeaxanthin, beta-cryptoxanthin), retinol, and tocopherols (alpha-tocopherol and gamma-tocopherol) are decreased in women with preeclampsia [18].
• Between July 2001 and July 2002, 1,178 housemaids (961 of them work in the top five most affluent districts of Lima, a metropolis of 8 million inhabitants considered nonendemic for cysticercosis) were evaluated for serum antibodies to Taenia solium and stool microscopy for taeniasis and cysticercosis [19].
• Before coronary occlusion and 1, 30, 60, and 90 min after reperfusion with the LIMA graft, coronary sinus (CS) blood was sampled to determine intraoperative myocardial ischemia (pH, lactate, pO2) and oxidative stress (malondialdehyde, MDA) [20].

Biological context of LIMA1

• EPLIN has at least two actin binding sites [21].
• EPLIN does not affect the kinetics of spontaneous actin polymerization or elongation at the barbed end, but inhibits branching nucleation of actin filaments by Arp2/3 complex [21].
• The amino acid sequence of EPLIN is characterized by the presence of a single centrally located LIM domain [1].
• Both EPLIN isoforms localize to filamentous actin and suppress cell proliferation when overexpressed [1].
• The DNA sequences flanking the EPLIN-alpha or EPLIN-beta transcriptional start sites were capable of stimulating the expression of promoter reporter constructs [2].

Anatomical context of LIMA1

• Purified recombinant EPLIN inhibits actin filament depolymerization and cross-links filaments in bundles [21].
• EPLIN increases the number and size of actin stress fibers and inhibits membrane ruffling induced by Rac [21].
• We have identified a novel cytoskeletal protein, EPLIN (Epithelial Protein Lost In Neoplasm), that is preferentially expressed in human epithelial cells [1].
• Two EPLIN isoforms, a 600 amino acid EPLIN-alpha and a 759 amino acid EPLIN-beta, are detected in primary epithelial cells of oral mucosa, prostate and mammary glands [1].
• EPLIN is a novel LIM domain protein that co-localizes to the actin stress fibers and focal adhesion plaques [2].

Associations of LIMA1 with chemical compounds

• In a peri-urban shanty town in Lima, Peru, that was hyperendemic for Giardia lamblia, 44 children aged between 0.9 months and 10 years were effectively treated for Giardia lamblia with tinidazole [22].
• Fluorescence polarization decay of tyrosine in lima bean trypsin inhibitor [23].
• Additionally, it was inhibited by popcorn inhibitor, leupeptin, hexadimethrine bromide, protamine sulfate, dansyl-arginine N-(3-ethyl-1,5-pentanediyl) amide (DAPA), diisopropylphosphofluoridate (DFP), aprotinin, and at excessively high concentrations, soybean and lima bean trypsin inhibitors [24].
• In general, the enzymatically active carboxy-terminal fragment of HF (HFf) was inhibited by the same agents that inhibited HFea, but aprotinin, protamine sulfate and hexadimethrine bromide were more effective against HFf than HFea, while the reverse was true of lima bean trypsin inhibitor [24].
• Antibody-induced cell detachment was partially inhibited by the addition of diisopropylfluorophosphate (DFP), and detachment was completely inhibited by the addition of lima bean trypsin inhibitor [25].

Other interactions of LIMA1

• Epithelial protein lost in neoplasm (EPLIN) is a cytoskeleton-associated protein encoded by a gene that is down-regulated in transformed cells [21].

Analytical, diagnostic and therapeutic context of LIMA1

• We used the 13C-urea breath test to study the prevalence of infection in 407 Peruvian children from Lima, aged 2 months to 12 years, from families of low and high socioeconomic status [15].
• A simple one-step procedure has been developed to effect complete removal of trypsin, chymotrypsin, and chymotrypsinogen by a combination of affinity chromatography and salting-out adsorption on lima bean protease inhibitor coupled to Sepharose (a column (0.9 X 60 cm) operated in series with a regeneratable 1-ml bed) [26].
• However, despite the similar activities described, several significant differences between recombinant and native lima bean lectin were found, including mobility on gel filtration, aggregation in solution, and its CD spectrum [27].
• Northern blot analysis showed absence of lectin mRNA in the leaves, roots, or stems of 16-day-old lima bean plants [28].
• Southern blot analysis reveals the presence of several genes which hybridize to the cloned gene and which we propose are genes included in the lima bean lectin gene family [27].

References